ArticleBiomedical optics express2016
Multiphoton excited hemoglobin fluorescence and third harmonic generation for non-invasive microscopy of stored blood.
Article in Biomedical optics express, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed, 35 citations in OpenAlex.
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- Compact portable multiphoton microscopy reveals histopathological hallmarks of unprocessed lung tumor tissue in real time.Translational biophotonics · 2020Article
- Label-free multiphoton microscopy as a tool to investigate alterations of cerebral aneurysms.Scientific reports · 2020Article
- Label-free assessment of hemodynamics in individual cortical brain vessels using third harmonic generation microscopy.Biomedical optics express · 2020Article
- Report on fluorescence lifetime imaging using multiphoton laser scanning microscopy targeting sentinel lymph node diagnostics.Journal of biomedical optics · 2020Article
- Second and third harmonic generation microscopy visualizes key structural components in fresh unprocessed healthy human breast tissue.Journal of biophotonics · 2019Article
- Visualize and quantify the structural alteration of the rat spinal cord injury based on multiphoton microscopy.Lasers in medical science · 2019Article
- Characterizing stratum corneum structure, barrier function, and chemical content of human skin with coherent Raman scattering imaging.Biomedical optics express · 2018Article
- Dual-wavelength oblique back-illumination microscopy for the non-invasive imaging and quantification of blood in collection and storage bags.Biomedical optics express · 2018Article
- Widefield standing wave microscopy of red blood cell membrane morphology with high temporal resolution.Biomedical optics express · 2018Article
- Visualization of laser tattoo removal treatment effects in a mouse model by two-photon microscopy.Biomedical optics express · 2017Article
- Epi-direction detected multimodal imaging of an unstained mouse retina with a Yb-fiber laser.Proceedings of SPIE--the International Society for Optical Engineering · 2017Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Red blood cells (RBC) in two-photon excited fluorescence (TPEF) microscopy usually appear as dark disks because of their low fluorescent signal. Here we use 15fs 800nm pulses for TPEF, 45fs 1060nm pulses for three-photon excited fluorescence, and third harmonic generation (THG) imaging. We find sufficient fluorescent signal that we attribute to hemoglobin fluorescence after comparing time and wavelength resolved spectra of other expected RBC endogenous fluorophores: NADH, FAD, biliverdin, and bilirubin. We find that both TPEF and THG microscopy can be used to examine erythrocyte morphology non-invasively without breaching a blood storage bag.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.