ArticleScientific reports2016
Applying high-resolution melting (HRM) technology to identify five commonly used Artemisia species.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 51 citations in OpenAlex.
- Development of plastome-based HRM markers for the authentication of 12 major medicinal herbs in the Apiaceae family.Scientific reports · 2026Article
- Chloroplast mini-barcodes combined with high resolution melting analysis to identify herbal medicine Difengpi (Heliyon · 2024Article
- Plant-based nanoparticles targeting malaria management.Frontiers in pharmacology · 2024Review
- Distinguishing fanged frogs (Limnonectes) species (Amphibia: Anura: Dicroglossidae), from Thailand using high resolution melting analysis.Scientific reports · 2023Article
- Article
- Horizon scan of DNA-based methods for quality control and monitoring of herbal preparations.Frontiers in pharmacology · 2023Review
- Emerging biotechnology applications in natural product and synthetic pharmaceutical analyses.Acta pharmaceutica Sinica. B · 2022Review
- Genetic and chemical markers for authentication of three Artemisia species: A. capillaris, A. gmelinii, and A. fukudo.PloS one · 2022Article
- ReScribe: An Unrestrained Tool Combining Multiplex Recombineering and Minimal-PAM ScCas9 for Genome RecodingACS synthetic biology · 2021Article
- Differentiation of Mitragyna speciosa, a narcotic plant, from allied Mitragyna species using DNA barcoding-high-resolution melting (Bar-HRM) analysis.Scientific reports · 2021Article
- Integration of advanced technologies for plant variety and cultivar identification.Journal of biosciences · 2021Review
- Discriminating larvae of two syntopic Cychramus species (Coleoptera, Nitidulidae) by means of bar-HRM analysis.Molecular biology reports · 2020Article
- Comparative chloroplast genome analysis of Artemisia (Asteraceae) in East Asia: insights into evolutionary divergence and phylogenomic implications.BMC genomics · 2020Article
- Multiplex Real-Time PCR with HRM for Detection ofFood technology and biotechnology · 2019Article
- The Essential Oils and Eucalyptol FromFrontiers in pharmacology · 2019Article
- Mitigating the Impact of Admixtures in Thai Herbal Products.Frontiers in pharmacology · 2019Review
- Article
- Bar-HRM for authenticating soursop (Annona muricata) tea.Scientific reports · 2018Article
- Population authentication of the traditional medicinal plant Cassia tora L. based on ISSR markers and FTIR analysis.Scientific reports · 2018Article
- The development of functional mapping by three sex-related loci on the third whorl of different sex types of Carica papaya L.PloS one · 2018Article
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Authors and funding
5 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Many members of the genus Artemisia are important for medicinal purposes with multiple pharmacological properties. Often, these herbal plants sold on the markets are in processed forms so it is difficult to authenticate. Routine testing and identification of these herbal materials should be performed to ensure that the raw materials used in pharmaceutical products are suitable for their intended use. In this study, five commonly used Artemisia species included Artemisia argyi, Artemisia annua, Artemisia lavandulaefolia, Artemisia indica, and Artemisia atrovirens were analyzed using high resolution melting (HRM) analysis based on the internal transcribed spacer 2 (ITS2) sequences. The melting profiles of the ITS2 amplicons of the five closely related herbal species are clearly separated so that they can be differentiated by HRM method. The method was further applied to authenticate commercial products in powdered. HRM curves of all the commercial samples tested are similar to the botanical species as labeled. These congeneric medicinal products were also clearly separated using the neighbor-joining (NJ) tree. Therefore, HRM method could provide an efficient and reliable authentication system to distinguish these commonly used Artemisia herbal products on the markets and offer a technical reference for medicines quality control in the drug supply chain.
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