ArticleCold Spring Harbor protocols2016
Combining Optogenetics and Electrophysiology to Analyze Projection Neuron Circuits.
Article in Cold Spring Harbor protocols, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed, 32 citations in OpenAlex.
- Cortical dynamics in hand/forelimb S1 and M1 evoked by brief photostimulation of the mouse's hand.eLife · 2025Article
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- Increased Interhemispheric Connectivity of a Distinct Type of Hippocampal Pyramidal Cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2024Article
- A rabies virus-based toolkit for efficient retrograde labeling and monosynaptic tracing.Neural regeneration research · 2023Article
- Reward and aversion processing by input-defined parallel nucleus accumbens circuits in mice.Nature communications · 2022Article
- Neuropilin 2/Plexin-A3 Receptors Regulate the Functional Connectivity and the Excitability in the Layers 4 and 5 of the Cerebral Cortex.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2022Article
- Article
- Circuit organization of the excitatory sensorimotor loop through hand/forelimb S1 and M1.eLife · 2021Article
- The Parental Dilemma: How Evolution of Diverse Strategies for Infant Care Informs Social Behavior Circuits.Frontiers in neural circuits · 2021Article
- Cortico-Thalamo-Cortical Circuits of Mouse Forelimb S1 Are Organized Primarily as Recurrent Loops.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2020Article
- Article
- Anterolateral Motor Cortex Connects with a Medial Subdivision of Ventromedial Thalamus through Cell Type-Specific Circuits, Forming an Excitatory Thalamo-Cortico-Thalamic Loop via Layer 1 Apical Tuft Dendrites of Layer 5B Pyramidal Tract Type Neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2018Article
- The State of the NIH BRAIN Initiative.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2018Article
- Rational design of silicon structures for optically controlled multiscale biointerfaces.Nature biomedical engineering · 2018Article
- A Corticocortical Circuit Directly Links Retrosplenial Cortex to M2 in the Mouse.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
A set of methods is described for channelrhodopsin-2 (ChR2)-based synaptic circuit analysis that combines photostimulation of virally transfected presynaptic neurons' axons with whole-cell electrophysiological recordings from retrogradely labeled postsynaptic neurons. The approach exploits the preserved photoexcitability of ChR2-expressing axons in brain slices and can be used to assess either local or long-range functional connections. Stereotaxic injections are used both to express ChR2 selectively in presynaptic axons of interest (using rabies virus [RV] or adeno-associated virus [AAV]) and to label two types of postsynaptic projection neurons of interest with fluorescent retrograde tracers. In brain slices, tracer-labeled postsynaptic neurons are targeted for whole-cell electrophysiological recordings, and synaptic connections are assessed by sampling voltage or current responses to light-emitting diode (LED) photostimulation of ChR2-expressing axons. The data are analyzed to estimate the relative amplitude of synaptic input and other connectivity parameters. Pharmacological and electrophysiological manipulations extend the versatility of the basic approach, allowing the dissection of monosynaptic versus disynaptic responses, excitatory versus inhibitory responses, and more. The method enables rapid, quantitative characterization of synaptic connectivity between defined pre- and postsynaptic classes of neurons.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.