ArticleProceedings of the National Academy of Sciences of the United States of America2016
An evolutionarily conserved element in initiator tRNAs prompts ultimate steps in ribosome maturation.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 49 citations in OpenAlex.
- Mechanisms and disease relevance of mitochondrial translation in humans.Nature reviews. Molecular cell biology · 2026Review
- Role of the anti-Shine-Dalgarno sequence of 16S rRNA in Flavobacterium johnsoniae.Nucleic acids research · 2026Article
- The Conserved GTPase LepA May Contribute to the Final Proper Stabilization of the 3' Domain of the 30S Subunit During Ribosome Assembly.International journal of molecular sciences · 2026Article
- A complex between IF2 and NusA suggests early coupling of transcription-translation.Nature communications · 2025Article
- Coupling of ribosome biogenesis and translation initiation in human mitochondria.Nature communications · 2025Article
- Cys-tRNAj as a Second Translation Initiator for Priming Proteins with Cysteine in Bacteria.ACS omega · 2025Article
- Physiological significance of the two isoforms of initiator tRNAs inJournal of bacteriology · 2024Article
- Lamotrigine-mediated rescue of RsgA-deficientJournal of bacteriology · 2024Article
- Late consolidation of rRNA structure during co-transcriptional assembly inbioRxiv : the preprint server for biology · 2024Article
- GTPase Era at the heart of ribosome assembly.Frontiers in molecular biosciences · 2023Review
- Lamotrigine compromises the fidelity of initiator tRNA recruitment to the ribosomal P-site by IF2 and the RbfA release from 30S ribosomes inRNA biology · 2023Article
- The initiation factor 3 (IF3) residues interacting with initiator tRNA elbow modulate the fidelity of translation initiation and growth fitness in Escherichia coli.Nucleic acids research · 2022Article
- The impact of mistranslation on phenotypic variability and fitness.Evolution; international journal of organic evolution · 2021Article
- Functional Analysis of BipA in E. coli Reveals the Natural Plasticity of 50S Subunit Assembly.Journal of molecular biology · 2020Article
- RbfA and IF3 couple ribosome biogenesis and translation initiation to increase stress tolerance.Nucleic acids research · 2020Article
- Ribosome assembly defects subvert initiation Factor3 mediated scrutiny of bona fide start signal.Nucleic acids research · 2019Article
- Interplay of cold shock protein E with an uncharacterized protein, YciF, lowers porin expression and enhances bile resistance inThe Journal of biological chemistry · 2019Article
- Rapid formylation of the cellular initiator tRNA population makes a crucial contribution to its exclusive participation at the step of initiation.Nucleic acids research · 2019Article
- Sustenance of Escherichia coli on a single tRNAMet.Nucleic acids research · 2018Article
- Role of ribosome assembly in Escherichia coli ribosomal RNA degradation.Nucleic acids research · 2018Article
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ribosome biogenesis, a complex multistep process, results in correct folding of rRNAs, incorporation of >50 ribosomal proteins, and their maturation. Deficiencies in ribosome biogenesis may result in varied faults in translation of mRNAs causing cellular toxicities and ribosomopathies in higher organisms. How cells ensure quality control in ribosome biogenesis for the fidelity of its complex function remains unclear. Using Escherichia coli, we show that initiator tRNA (i-tRNA), specifically the evolutionarily conserved three consecutive GC base pairs in its anticodon stem, play a crucial role in ribosome maturation. Deficiencies in cellular contents of i-tRNA confer cold sensitivity and result in accumulation of ribosomes with immature 3' and 5' ends of the 16S rRNA. Overexpression of i-tRNA in various strains rescues biogenesis defects. Participation of i-tRNA in the first round of initiation complex formation licenses the final steps of ribosome maturation by signaling RNases to trim the terminal extensions of immature 16S rRNA.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.