ArticleInfection and immunity2016
Genetic Determinants of Salmonella enterica Serovar Typhimurium Proliferation in the Cytosol of Epithelial Cells.
Article in Infection and immunity, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 34 citations in OpenAlex.
- Host-microbiota-pathogen interactions drive Salmonella enterica serovar Typhimurium pathogenesis.Nature reviews. Microbiology · 2026Review
- The Manipulation of Host Cells byInternational journal of molecular sciences · 2026Review
- Controlling intracellular protein delivery, tumor colonization and tissue distribution using flhDC in clinically relevant ΔsseJ Salmonella.Molecular therapy : the journal of the American Society of Gene Therapy · 2025Article
- Population structure and ongoing microevolution of the emerging multidrug-resistant Salmonella Typhimurium ST213.npj antimicrobials and resistance · 2024Article
- A genome-wide collection of barcoded single-gene deletion mutants in Salmonella enterica serovar Typhimurium.PloS one · 2024Article
- Essential role for epithelial HIF-mediated xenophagy in control of Salmonella infection and dissemination.Cell reports · 2022Article
- Article
- Salmonella Typhimurium outer membrane protein A (OmpA) renders protection from nitrosative stress of macrophages by maintaining the stability of bacterial outer membrane.PLoS pathogens · 2022Article
- Fit to dwell in many places - The growing diversity of intracellularFrontiers in cellular and infection microbiology · 2022Review
- Intracellular niche-specific profiling reveals transcriptional adaptations required for the cytosolic lifestyle of Salmonella enterica.PLoS pathogens · 2021Article
- Sulfate Import inInfection and immunity · 2021Article
- Article
- Article
- Genetic Determinants ofApplied and environmental microbiology · 2020Article
- The motility regulator flhDC drives intracellular accumulation and tumor colonization of Salmonella.Journal for immunotherapy of cancer · 2019Article
- A macrophage-based screen identifies antibacterial compounds selective for intracellular Salmonella Typhimurium.Nature communications · 2019Article
- Evaluation of Incompatibility Group I1 (IncI1) Plasmid-ContainingFrontiers in veterinary science · 2019Article
- Controlled Activity of themBio · 2017Article
- Review
- RNA-Seq unveils new attributes of the heterogeneous Salmonella-host cell communication.RNA biology · 2017Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Intestinal epithelial cells provide an important colonization niche for Salmonella enterica serovar Typhimurium during gastrointestinal infections. In infected epithelial cells, a subpopulation of S Typhimurium bacteria damage their internalization vacuole, leading to escape from the Salmonella-containing vacuole (SCV) and extensive proliferation in the cytosol. Little is known about the bacterial determinants of nascent SCV lysis and subsequent survival and replication of Salmonella in the cytosol. To pinpoint S Typhimurium virulence factors responsible for these steps in the intracellular infectious cycle, we screened a S Typhimurium multigene deletion library in Caco-2 C2Bbe1 and HeLa epithelial cells for mutants that had an altered proportion of cytosolic bacteria compared to the wild type. We used a gentamicin protection assay in combination with a chloroquine resistance assay to quantify total and cytosolic bacteria, respectively, for each strain. Mutants of three S Typhimurium genes, STM1461 (ydgT), STM2829 (recA), and STM3952 (corA), had reduced cytosolic proliferation compared to wild-type bacteria, and one gene, STM2120 (asmA), displayed increased cytosolic replication. None of the mutants were affected for lysis of the nascent SCV or vacuolar replication in epithelial cells, indicating that these genes are specifically required for survival and proliferation of S Typhimurium in the epithelial cell cytosol. These are the first genes identified to contribute to this step of the S Typhimurium infectious cycle.
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