ArticleDevelopment (Cambridge, England)2016
Reversible optogenetic control of kinase activity during differentiation and embryonic development.
Article in Development (Cambridge, England), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
- Protocol to determine oligomerization states of proteins in living cells using photon counting histogram analysis.STAR protocols · 2026Article
- Lights up on the embryonic dance: tools and applications of optogenetics in developmental biology.Genes & development · 2026Review
- Dynamic control of Raf-ERK signaling modulates neuronal activity across biological scales.bioRxiv : the preprint server for biology · 2026Article
- Resolving oligomeric states of photoactivatable proteins in living cells via photon counting histogram analysis.iScience · 2025Article
- Development of an optogenetics tool, Opto-RANK, for control of osteoclast differentiation using blue light.Scientific reports · 2024Article
- Optogenetic Signaling Activation in Zebrafish Embryos.Journal of visualized experiments : JoVE · 2023Article
- Article
- Precise modulation of embryonic development through optogenetics.Genesis (New York, N.Y. : 2000) · 2022Review
- Optophysiology: Illuminating cell physiology with optogenetics.Physiological reviews · 2022Review
- Optogenetic Application to Investigating Cell Behavior and Neurological Disease.Frontiers in cellular neuroscience · 2022Review
- Extracellular Optogenetics at the Interface of Synthetic Biology and Materials Science.Frontiers in bioengineering and biotechnology · 2022Review
- 2A and 2A-like Sequences: Distribution in Different Virus Species and Applications in Biotechnology.Viruses · 2021Review
- Optogenetic Control of the Canonical Wnt Signaling Pathway During Xenopus laevis Embryonic Development.Journal of molecular biology · 2021Article
- Optobiochemistry: Genetically Encoded Control of Protein Activity by Light.Annual review of biochemistry · 2021Review
- Optogenetic Approaches for the Spatiotemporal Control of Signal Transduction Pathways.International journal of molecular sciences · 2021Review
- Optogenetic delivery of trophic signals in a genetic model of Parkinson's disease.PLoS genetics · 2021Article
- Modulating organelle distribution using light-inducible heterodimerization inSTAR protocols · 2021Article
- Reverse and Forward Engineering Multicellular Structures with Optogenetics.Current opinion in biomedical engineering · 2020Article
- Article
- A Generalizable Optogenetic Strategy to Regulate Receptor Tyrosine Kinases during Vertebrate Embryonic Development.Journal of molecular biology · 2020Article
Corrections and comments
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Authors and funding
11 authors.
Funding
Abstract
A limited number of signaling pathways are repeatedly used to regulate a wide variety of processes during development and differentiation. The lack of tools to manipulate signaling pathways dynamically in space and time has been a major technical challenge for biologists. Optogenetic techniques, which utilize light to control protein functions in a reversible fashion, hold promise for modulating intracellular signaling networks with high spatial and temporal resolution. Applications of optogenetics in multicellular organisms, however, have not been widely reported. Here, we create an optimized bicistronic optogenetic system using Arabidopsis thaliana cryptochrome 2 (CRY2) protein and the N-terminal domain of cryptochrome-interacting basic-helix-loop-helix (CIBN). In a proof-of-principle study, we develop an optogenetic Raf kinase that allows reversible light-controlled activation of the Raf/MEK/ERK signaling cascade. In PC12 cells, this system significantly improves light-induced cell differentiation compared with co-transfection. When applied to Xenopus embryos, this system enables blue light-dependent reversible Raf activation at any desired developmental stage in specific cell lineages. Our system offers a powerful optogenetic tool suitable for manipulation of signaling pathways with high spatial and temporal resolution in a wide range of experimental settings.
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Registered trials
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