Evidence map›Paper›PMID 27697785›Full record

ArticleJournal of experimental botany2016

A flow cytometric method for estimating S-phase duration in plants.

Leigh Mickelson-Young, Emily Wear, Patrick Mulvaney, Tae-Jin Lee, Eric S Szymanski, George Allen, Linda Hanley-Bowdoin, William Thompson

Abstract read
In one paragraph

Article in Journal of experimental botany, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed.

  1. Article
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  3. Review
  4. Article
  5. The Role of Topoisomerase II in DNA Repair and Recombination inInternational journal of molecular sciences · 2021
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  6. Review
  7. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Leigh Mickelson-YoungDepartment of Plant and Microbial Biology, North Carolina State University, Raleigh, NC 27695, USA lamickel@ncsu.edu.
Emily WearDepartment of Plant and Microbial Biology, North Carolina State University, Raleigh, NC 27695, USA.
Patrick MulvaneyDepartment of Plant and Microbial Biology, North Carolina State University, Raleigh, NC 27695, USA.
Tae-Jin LeeDepartment of Horticultural Science, North Carolina State University, Raleigh, NC 27695, USA.
Eric S SzymanskiDepartment of Plant and Microbial Biology, North Carolina State University, Raleigh, NC 27695, USA.
George AllenDepartment of Horticultural Science, North Carolina State University, Raleigh, NC 27695, USA.
Linda Hanley-BowdoinDepartment of Plant and Microbial Biology, North Carolina State University, Raleigh, NC 27695, USA.
William ThompsonDepartment of Plant and Microbial Biology, North Carolina State University, Raleigh, NC 27695, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The duration of the DNA synthesis stage (S phase) of the cell cycle is fundamental in our understanding of cell cycle kinetics, cell proliferation, and DNA replication timing programs. Most S-phase duration estimates that exist for plants are based on indirect measurements. We present a method for directly estimating S-phase duration by pulse-labeling root tips or actively dividing suspension cells with the halogenated thymidine analog 5-ethynl-2'-deoxyuridine (EdU) and analyzing the time course of replication with bivariate flow cytometry. The transition between G

Indexed as

S PhaseArabidopsisDeoxyuridineDNA, PlantFlow CytometryG1 PhaseG2 PhaseHordeumMeristemOryzaTriticumZea mays5-ethynyl-2'-deoxyuridineDeoxyuridineDNA, PlantArabidopsisbarleyEdUflow cytometrymaizereplication timingriceS-phase durationwheat.

Identifiers

PMID27697785
PMCPMC5100020

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.