ArticleMolecular microbiology2017
The Paracoccus denitrificans NarK-like nitrate and nitrite transporters-probing nitrate uptake and nitrate/nitrite exchange mechanisms.
Article in Molecular microbiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 37 citations in OpenAlex.
- Kinetic plasticity of nitrite-oxidizing bacteria containing cytoplasmic nitrite oxidoreductase.The ISME journal · 2026Article
- A Key Metabolic Protein in Active Mycobacterium tuberculosis: Insights into Carbon, Nitrogen, and Sulfur Metabolism.Advances in experimental medicine and biology · 2026Review
- Nitrate reduction salvage pathway inFrontiers in microbiology · 2026Article
- Dissimilatory nitrate reductions in soil Neobacillus and Bacillus strains under aerobic condition.Journal of microbiology (Seoul, Korea) · 2025Article
- Mechanisms underlying the low-temperature adaptation of 17β-estradiol-degrading bacterial strainFrontiers in microbiology · 2024Article
- Partnered Excited-State Intermolecular Proton Transfer Fluorescence (P-ESIPT) Signaling for Nitrate Sensing and High-Resolution Cell-Imaging.Molecules (Basel, Switzerland) · 2022Article
- Coculture ofMicroorganisms · 2022Article
- Nutrient Limitation Causes Differential Expression of Transport- and Metabolism Genes in the Compartmentalized Anammox BacteriumFrontiers in microbiology · 2020Article
- Controls on the Isotopic Composition of Nitrite (δScientific reports · 2019Article
- A dual functional redox enzyme maturation protein for respiratory and assimilatory nitrate reductases in bacteria.Molecular microbiology · 2019Article
- A new family of nitrate/nitrite transporters involved in denitrification.International microbiology : the official journal of the Spanish Society for Microbiology · 2019Article
- Community Structure of Bacteria Associated With DriftingFrontiers in microbiology · 2019Article
- Transcriptional and translational adaptation to aerobic nitrate anabolism in the denitrifierThe Biochemical journal · 2017Article
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Authors and funding
9 authors at 5 institutions in 2 countries.
Funding
Abstract
Nitrate and nitrite transport across biological membranes is often facilitated by protein transporters that are members of the major facilitator superfamily. Paracoccus denitrificans contains an unusual arrangement whereby two of these transporters, NarK1 and NarK2, are fused into a single protein, NarK, which delivers nitrate to the respiratory nitrate reductase and transfers the product, nitrite, to the periplasm. Our complementation studies, using a mutant lacking the nitrate/proton symporter NasA from the assimilatory nitrate reductase pathway, support that NarK1 functions as a nitrate/proton symporter while NarK2 is a nitrate/nitrite antiporter. Through the same experimental system, we find that Escherichia coli NarK and NarU can complement deletions in both narK and nasA in P. denitrificans, suggesting that, while these proteins are most likely nitrate/nitrite antiporters, they can also act in the net uptake of nitrate. Finally, we argue that primary sequence analysis and structural modelling do not readily explain why NasA, NarK1 and NarK2, as well as other transporters from this protein family, have such different functions, ranging from net nitrate uptake to nitrate/nitrite exchange.
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