ArticlePharmacognosy research
Kayeassamin A Isolated from the Flower of
Article in Pharmacognosy research. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 13 citations in OpenAlex.
- Total Synthesis of Kayeassamin A and Mammeasin A: Overcoming Challenges in the Construction of Sterically Overcrowded Pentasubstituted Coumarin Frameworks.The Journal of organic chemistry · 2026Article
- Article
- Structures of Mammeasins P and Q, Coumarin-Related Polysubstituted Benzofurans, from the Thai Medicinal PlantPharmaceuticals (Basel, Switzerland) · 2023Article
- Phytochemicals with Chemopreventive Activity Obtained from the Thai Medicinal PlantInternational journal of molecular sciences · 2022Article
- Geranylated Coumarins From Thai Medicinal PlantFrontiers in chemistry · 2020Article
- Rutamarin, an Active Constituent fromPharmacognosy magazine · 2017Article
- Withaferin-A Induces Apoptosis in Osteosarcoma U2OS Cell Line via Generation of ROS and Disruption of Mitochondrial Membrane Potential.Pharmacognosy magazineArticle
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
background
objectiveIn this study, we investigated the effect of KA on cell viability and apoptotic mechanisms in HL-60 human leukemia cells. MATERIALS AND
methodsCell viability was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Nuclear morphology and DNA fragmentation were observed using Hoechst 33258 staining and agarose gel electrophoresis, respectively. The sub-G1 phase of cells was analyzed by flow cytometry after the cellular DNA had been stained with propidium iodide. The protein levels of poly (ADP-ribose) polymerase (PARP) and caspases were determined by Western blotting.
resultsKA exhibited a significant cytotoxic effect in a dose- and time-dependent manner, and induced chromatin condensation, DNA fragmentation, and sub-G1 phase DNA content, known as molecular events associated with the induction of apoptosis. In addition, KA strongly induced the activation of PARP and caspase-3 and -8, with weak caspase-9 activation. Furthermore, KA-induced DNA fragmentation was abolished by pretreatment with z-VAD-FMK (a broad caspase inhibitor), z-DEVD-FMK (a caspase-3 inhibitor), and z-IETD-FMK (a caspase-8 inhibitor), but not by z-LEHD-FMK (a caspase-9 inhibitor) pretreatment.
conclusionThese results indicate that KA triggers apoptotic cell death by activation of caspase-3 and -8 in HL-60 cells. SUMMARY: Kayeassamin A (KA) isolated from the flower of
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.