ArticleScientific reports2016
Inhibition of Rac1 GTPase activity affects porcine oocyte maturation and early embryo development.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 18 citations in OpenAlex.
- RAC1 signaling in polyploid giant cancer cells: Implications for tumorigenesis and therapy resistance.Cancer letters · 2026Review
- From Cytoskeletal Remodeling to Oocyte Quality: The Emerging Role of Mechanics.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Rab32-based vesicles coordinate mitochondria and actin for spindle migration and organelle rearrangement in oocyte meiosis.Journal of advanced research · 2026Article
- Mevalonate metabolites boost aged oocyte quality through prenylation of small GTPases.Nature aging · 2025Article
- Extracellular osmolarity regulates osteoblast migration through the TRPV4-Rho/ROCK signaling.Communications biology · 2025Article
- NET1 is a critical regulator of spindle assembly and actin dynamics in mouse oocytes.Reproductive biology and endocrinology : RB&E · 2024Article
- Effects of PAMK on lncRNA, miRNA, and mRNA expression profiles of thymic epithelial cells.Functional & integrative genomics · 2022Article
- LRRK2 regulates actin assembly for spindle migration and mitochondrial function in mouse oocyte meiosis.Journal of molecular cell biology · 2022Article
- Apoptotic cells in mouse blastocysts are eliminated by neighbouring blastomeres.Scientific reports · 2021Article
- "Biomechanical Signaling in Oocytes and Parthenogenetic Cells".Frontiers in cell and developmental biology · 2021Review
- Involvement of LIMK1/2 in actin assembly during mouse embryo development.Cell cycle (Georgetown, Tex.) · 2018Article
- Protein phosphatase 2A regulatory subunit B55α functions in mouse oocyte maturation and early embryonic development.Oncotarget · 2017Article
- Cytoskeleton-associated protein 5 and clathrin heavy chain binding regulates spindle assembly in mouse oocytes.Oncotarget · 2017Article
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mammalian oocyte asymmetric division relies on the eccentric positioning of the spindle, resulting in the polar body formation. Small signaling G protein Rac1 is a member of GTPases, which regulates a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. However, effects of Rac1 on the porcine oocyte maturation and early embryo development are not fully understood. In present study we investigated the role of Rac1 in oocyte maturation and embryo cleavage. We first found that Rac1 localized at the cortex of the porcine oocytes, and disrupting the Rac1 activities by treating with NSC 23766 led to the failure of polar body emission. In addition, a majority of treated oocytes exhibited abnormal spindle morphology, indicating that Rac1 may involve into porcine oocyte spindle formation. This might be due to the regulation of Rac1 on MAPK, since p-MAPK expression decreased after NSC 23766 treatments. Moreover, we found that the position of most meiotic spindles in treated oocytes were away from the cortex, indicating the roles of Rac1 on meiotic spindle positioning. Our results also showed that inhibition of Rac1 activity caused the failure of early embryo development. Therefore, our study showed the critical roles of Rac1 GTPase on porcine oocyte maturation and early embryo cleavage.
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