ArticleNature chemical biology2016
Functional mining of transporters using synthetic selections.
Article in Nature chemical biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 73 citations in OpenAlex.
- Genetically encoded biosensor enabled mining, characterisation and engineering of aromatic acid MFS transporters.Journal of biological engineering · 2025Article
- A previously unidentified sugar transporter for engineering of high-yield Streptomyces.Applied microbiology and biotechnology · 2024Article
- Soil microbiome characterization and its future directions with biosensing.Journal of biological engineering · 2024Article
- The Y-ome Conundrum: Insights into Uncharacterized Genes and Approaches for Functional Annotation.Molecular and cellular biochemistry · 2024Review
- Identification of transporters involved in aromatic compounds tolerance through screening of transporter deletion libraries.Microbial biotechnology · 2024Article
- Transporter annotations are holding up progress in metabolic modeling.Frontiers in systems biology · 2024Article
- Review
- Mining and engineering exporters for titer improvement of macrolide biopesticides in Streptomyces.Microbial biotechnology · 2022Article
- Identification and Engineering of Transporters for Efficient Melatonin Production inFrontiers in microbiology · 2022Article
- Identification of Glycoside Transporters From the Human Gut Microbiome.Frontiers in microbiology · 2022Article
- Microbial Plankton Community Structure and Function Responses to Vitamin BApplied and environmental microbiology · 2021Article
- Transporter engineering in microbial cell factories: the ins, the outs, and the in-betweens.Current opinion in biotechnology · 2020Review
- Article
- Article
- B vitamin acquisition by gut commensal bacteria.PLoS pathogens · 2020Article
- Functional metagenomics-guided discovery of potent Cas9 inhibitors in the human microbiome.eLife · 2019Article
- Overcoming genetic heterogeneity in industrial fermentations.Nature biotechnology · 2019Review
- Genetically encoded biosensors for lignocellulose valorization.Biotechnology for biofuels · 2019Review
- Cysteine-mediated decyanation of vitamin B12 by the predicted membrane transporter BtuM.Nature communications · 2018Article
- The evolving interface between synthetic biology and functional metagenomics.Nature chemical biology · 2018Review
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Only 25% of bacterial membrane transporters have functional annotation owing to the difficulty of experimental study and of accurate prediction of their function. Here we report a sequence-independent method for high-throughput mining of novel transporters. The method is based on ligand-responsive biosensor systems that enable selective growth of cells only if they encode a ligand-specific importer. We developed such a synthetic selection system for thiamine pyrophosphate and mined soil and gut metagenomes for thiamine-uptake functions. We identified several members of a novel class of thiamine transporters, PnuT, which is widely distributed across multiple bacterial phyla. We demonstrate that with modular replacement of the biosensor, we could expand our method to xanthine and identify xanthine permeases from gut and soil metagenomes. Our results demonstrate how synthetic-biology approaches can effectively be deployed to functionally mine metagenomes and elucidate sequence-function relationships of small-molecule transport systems in bacteria.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.