ArticleNucleic acids research2016
Single-molecule FRET reveals the pre-initiation and initiation conformations of influenza virus promoter RNA.
Article in Nucleic acids research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 39 citations in OpenAlex.
- Febrile temperature activates the innate immune response by promoting aberrant influenza A virus RNA synthesis.Science advances · 2026Article
- Cryo-EM structure of influenza polymerase bound to the cRNA promoter provides insights into the mechanism of viral replication.Communications biology · 2025Article
- Febrile temperature activates the innate immune response by promoting aberrant influenza A virus RNA synthesis.bioRxiv : the preprint server for biology · 2025Article
- Mutagenesis studies suggest a mechanism for influenza polymerase stalling during polyadenylation.Nucleic acids research · 2025Article
- Review
- The dynamic, motile and deformative properties of RNA nanoparticles facilitate the third milestone of drug development.Advanced drug delivery reviews · 2022Review
- The Host Factor ANP32A Is Required for Influenza A Virus vRNA and cRNA Synthesis.Journal of virology · 2022Article
- Determination of the vRNA and cRNA promoter activity by M segment-specific non-coding nucleotides of influenza A virus.RNA biology · 2021Article
- Review
- Structure and Function of the Influenza Virus Transcription and Replication Machinery.Cold Spring Harbor perspectives in medicine · 2020Review
- Identification of a Type-Specific Promoter Element That Differentiates between Influenza A and B Viruses.Journal of virology · 2019Article
- Substrate conformational dynamics facilitate structure-specific recognition of gapped DNA by DNA polymerase.Nucleic acids research · 2019Article
- Rapid functionalisation and detection of viruses via a novel CaScientific reports · 2019Article
- Real-time analysis of single influenza virus replication complexes reveals large promoter-dependent differences in initiation dynamics.Nucleic acids research · 2019Article
- Flu transcription captured in action.Nature structural & molecular biology · 2019Article
- Structural snapshots of actively transcribing influenza polymerase.Nature structural & molecular biology · 2019Article
- Mini viral RNAs act as innate immune agonists during influenza virus infection.Nature microbiology · 2018Article
- Single-molecule fluorescence imaging: Generating insights into molecular interactions in virology.Journal of biosciences · 2018Review
- A Mechanism for Priming and Realignment during Influenza A Virus Replication.Journal of virology · 2018Article
- Influenza A Virus Cell Entry, Replication, Virion Assembly and Movement.Frontiers in immunology · 2018Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 3 countries.
Funding
Abstract
Influenza viruses have a segmented viral RNA (vRNA) genome, which is replicated by the viral RNA-dependent RNA polymerase (RNAP). Replication initiates on the vRNA 3' terminus, producing a complementary RNA (cRNA) intermediate, which serves as a template for the synthesis of new vRNA. RNAP structures show the 3' terminus of the vRNA template in a pre-initiation state, bound on the surface of the RNAP rather than in the active site; no information is available on 3' cRNA binding. Here, we have used single-molecule Förster resonance energy transfer (smFRET) to probe the viral RNA conformations that occur during RNAP binding and initial replication. We show that even in the absence of nucleotides, the RNAP-bound 3' termini of both vRNA and cRNA exist in two conformations, corresponding to the pre-initiation state and an initiation conformation in which the 3' terminus of the viral RNA is in the RNAP active site. Nucleotide addition stabilises the 3' vRNA in the active site and results in unwinding of the duplexed region of the promoter. Our data provide insights into the dynamic motions of RNA that occur during initial influenza replication and has implications for our understanding of the replication mechanisms of similar pathogenic viruses.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.