ArticleStem cell reports2016
Differentiation of Human Embryonic Stem Cells to Endothelial Progenitor Cells on Laminins in Defined and Xeno-free Systems.
Article in Stem cell reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Protocol for PI3K inhibitor-free differentiation and maturation of human iPSC-derived arterial- and venous-like endothelial cells.STAR protocols · 2026Article
- Development of self-healing hydrogels to support choroidal endothelial cell transplantation for the treatment of early age related macular degeneration.Acta biomaterialia · 2025Article
- Pluripotent stem cell-derived committed cardiac progenitors remuscularize damaged ischemic hearts and improve their function in pigs.NPJ Regenerative medicine · 2023Article
- Definitive Endodermal Cells Supply an in vitro Source of Mesenchymal Stem/Stromal Cells.Communications biology · 2023Article
- Photoreceptor laminin drives differentiation of human pluripotent stem cells to photoreceptor progenitors that partially restore retina function.Molecular therapy : the journal of the American Society of Gene Therapy · 2023Article
- Extracellular matrix cues regulate the differentiation of pluripotent stem cell-derived endothelial cells.Frontiers in cardiovascular medicine · 2023Review
- Endothelial progenitor cells derived from embryonic stem cells prevent alveolar simplification in a murine model of bronchopulmonary dysplasia.Frontiers in cell and developmental biology · 2023Article
- Differentiation and molecular characterization of endothelial progenitor and vascular smooth muscle cells from induced pluripotent stem cells.BioImpacts : BI · 2023Article
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- The Differentiation of Pluripotent Stem Cells towards Endothelial Progenitor Cells - Potential Application in Pulmonary Arterial Hypertension.International journal of stem cells · 2022Article
- Leveraging interacting signaling pathways to robustly improve the quality and yield of human pluripotent stem cell-derived hepatoblasts and hepatocytes.Stem cell reports · 2022Article
- A High-Efficiency Method for the Production of Endothelial Cells from Human Induced Pluripotent Stem Cells.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Therapeutic Potential of Endothelial Progenitor Cells in Pulmonary Diseases.American journal of respiratory cell and molecular biology · 2021Review
- Extracellular Matrix-Based Biomaterials for Cardiovascular Tissue Engineering.Journal of cardiovascular development and disease · 2021Review
- Directed Differentiation of Hemogenic Endothelial Cells from Human Pluripotent Stem Cells.Journal of visualized experiments : JoVE · 2021Article
- From Human Pluripotent Stem Cells to 3D Cardiac Microtissues: Progress, Applications and Challenges.Bioengineering (Basel, Switzerland) · 2020Review
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- Von Willebrand Disease: From In Vivo to In Vitro Disease Models.HemaSphere · 2019Review
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A major hurdle for in vitro culturing of primary endothelial cells (ECs) is that they readily dedifferentiate, hampering their use for therapeutic applications. Human embryonic stem cells (hESCs) may provide an unlimited cell source; however, most current protocols deriving endothelial progenitor cells (EPCs) from hESCs use direct differentiation approaches albeit on undefined matrices, yet final yields are insufficient. We developed a method to culture monolayer hESCs on stem cell niche laminin (LN) LN511 or LN521 matrix. Here, we report a chemically defined, xeno-free protocol for differentiation of hESCs to EPCs using LN521 as the main culture substrate. We were able to generate ∼95% functional EPCs defined as VEGFR2
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