ArticlePLoS pathogens2016
A Novel Entry/Uncoating Assay Reveals the Presence of at Least Two Species of Viral Capsids During Synchronized HIV-1 Infection.
Article in PLoS pathogens, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed, 31 citations in OpenAlex.
- Article
- Direct translation of incoming retroviral genomes.Nature communications · 2024Article
- A snapshot on HIV-1 evolution through the identification of phylogenetic-specific properties of HIV-1 integrases M/O.PLoS pathogens · 2023Article
- Trim69 is a microtubule regulator that acts as a pantropic viral inhibitor.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Insights into HIV uncoating from single-particle imaging techniques.Biophysical reviews · 2022Review
- HIV-1 capsid variability: viral exploitation and evasion of capsid-binding molecules.Retrovirology · 2021Review
- The Role of Capsid in the Early Steps of HIV-1 Infection: New Insights into the Core of the Matter.Viruses · 2021Review
- Capsid Lattice Destabilization Leads to Premature Loss of the Viral Genome and Integrase Enzyme during HIV-1 Infection.Journal of virology · 2020Article
- Characterization of HIV-1 uncoating in human microglial cell lines.Virology journal · 2020Article
- IL-27 posttranslationally regulates Y-box binding protein-1 to inhibit HIV-1 replication in human CD4+ T cells.AIDS (London, England) · 2019Article
- A Novel Phenotype Links HIV-1 Capsid Stability to cGAS-Mediated DNA Sensing.Journal of virology · 2019Article
- Inhibitors of the HIV-1 capsid, a target of opportunity.Current opinion in HIV and AIDS · 2018Review
- Article
- Article
- An improved labeling strategy enables automated detection of single-virus fusion and assessment of HIV-1 protease activity in single virions.The Journal of biological chemistry · 2017Article
- Capsid-Dependent Host Factors in HIV-1 Infection.Trends in microbiology · 2017Review
- Early cytoplasmic uncoating is associated with infectivity of HIV-1.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Distinct functions of diaphanous-related formins regulate HIV-1 uncoating and transport.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Dynamics and regulation of nuclear import and nuclear movements of HIV-1 complexes.PLoS pathogens · 2017Article
- Phosphorylation of the HIV-1 capsid by MELK triggers uncoating to promote viral cDNA synthesis.PLoS pathogens · 2017Article
Corrections and comments
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Authors and funding
3 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To better characterize the behavior of HIV-1 capsids we developed EURT, for Entry/Uncoating assay based on core-packaged RNA availability and Translation. EURT is an alternative to Blam-Vpr, but as reporter RNA translation relies on core opening, it can be used to study viral capsids behavior. Our study reveals the existence of two major capsid species, a dead end one in which the viral genome is readily exposed to the cytoplasm and a functional one in which such exposure requires artificial core destabilization. Although reverse transcription drives a faster loss of susceptibility of viral cores to high doses of PF74, it does not lead to higher exposure of the viral genome, implying that viral cores protect the genome irrespectively of reverse transcription. Lastly, IFNα drifts cores from functional to non-functional species, revealing a novel core-destabilizing activity. This assay sheds new light on the behavior of viral cores inside target cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.