ArticleToxicology mechanisms and methods2016
Electronic cigarette aerosol induces significantly less cytotoxicity than tobacco smoke.
Article in Toxicology mechanisms and methods, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
46 citing papers in PubMed, 1 synthesis or guideline pooled it, 106 citations in OpenAlex.
- Pulmonary effects of e-liquid flavors: a systematic review.Journal of toxicology and environmental health. Part B, Critical reviews · 2022Pooled it
- Time since cigarette smoking cessation and self-rated health among exclusive e-cigarette users.Irish journal of medical science · 2026Article
- Evaluation of Acute Exposure to Combustible and Novel Tobacco Products Using an In Vitro Human Airway Organ Tissue Equivalent Model.Journal of applied toxicology : JAT · 2026Article
- Review
- Mechanistic toxicity profiling of nicotine-rich e-liquids: mitochondrial and oxidative stress responses in ALI-exposed bronchial cells.Molecular and cellular biochemistry · 2026Article
- Comparative toxicological assessment of cigarettes and new category products via anToxicology reports · 2024Article
- Characterization of mass distribution in a biomimetic aerosol exposure system.Inhalation toxicology · 2024Article
- In vitro toxicological evaluation of aerosols generated by a 4th generation vaping device using nicotine salts in an air-liquid interface system.Respiratory research · 2024Article
- Evaluation of Cytotoxicity and Oxidative Stress of Whole Aerosol from Vuse Alto ENDS Products.Toxics · 2024Article
- Challenges in current inhalable tobacco toxicity assessment models: A narrative review.Tobacco induced diseases · 2024Review
- Consideration of vaping products as an alternative to adult smoking: a narrative review.Substance abuse treatment, prevention, and policy · 2023Review
- Cytotoxicity, mutagenicity and genotoxicity of electronic cigarettes emission aerosols compared to cigarette smoke: the REPLICA project.Scientific reports · 2023Article
- Evaluation of the Effects of e-Cigarette Aerosol Extracts and Tobacco Cigarette Smoke Extracts on RAW264.7 Cells.ACS omega · 2023Article
- Heparan Sulfate and Enoxaparin Interact at the Interface of the Spike Protein of HCoV-229E but Not with HCoV-OC43.Viruses · 2023Article
- Electronic Cigarettes: A Narrative Review and Cohort Study of Electronic Cigarette Users in the Otolaryngology Clinic.The Permanente journal · 2022Review
- Considerations on dosimetry for in vitro assessment of e-cigarette toxicity.Respiratory research · 2022Review
- Chemical characterisation of the vapour emitted by an e-cigarette using a ceramic wick-based technology.Scientific reports · 2022Article
- Computational modeling method to estimate secondhand exposure potential from exhalations during e-vapor product use under various real-world scenarios.Internal and emergency medicine · 2022Article
- Article
- Electronic nicotine delivery systems exhibit reduced bronchial epithelial cells toxicity compared to cigarette: the Replica Project.Scientific reports · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Electronic cigarettes (E-cigarettes) are a potential means of addressing the harm to public health caused by tobacco smoking by offering smokers a less harmful means of receiving nicotine. As e-cigarettes are a relatively new phenomenon, there are limited scientific data on the longer-term health effects of their use. This study describes a robust in vitro method for assessing the cytotoxic response of e-cigarette aerosols that can be effectively compared with conventional cigarette smoke. This was measured using the regulatory accepted Neutral Red Uptake assay modified for air-liquid interface (ALI) exposures. An exposure system, comprising a smoking machine, traditionally used for in vitro tobacco smoke exposure assessments, was adapted for use with e-cigarettes to expose human lung epithelial cells at the ALI. Dosimetric analysis methods using real-time quartz crystal microbalances for mass, and post-exposure chemical analysis for nicotine, were employed to detect/distinguish aerosol dilutions from a reference Kentucky 3R4F cigarette and two commercially available e-cigarettes (Vype eStick and ePen). ePen aerosol induced 97%, 94% and 70% less cytotoxicity than 3R4F cigarette smoke based on matched EC
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.