ArticlePloS one
The Insulin-Like Proteins dILPs-2/5 Determine Diapause Inducibility in Drosophila.
Article in PloS one. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 63 citations in OpenAlex.
- The insulin and ecdysone pathways as regulators of diapause termination: transcriptional and protein insights from Pieris napi.BMC genomics · 2026Article
- Venom IMP-L2 from the EctoparasitoidInsects · 2025Article
- Cold-Sensing TRP Channels and Temperature Preference Modulate Ovarian Development in the Model OrganismInternational journal of molecular sciences · 2025Article
- Genome-wide identification of PAR domain protein 1 (PDP1) targets through ChIP-seq reveals the regulation of diapause-specific characteristics in Culex pipiens.Insect molecular biology · 2024Article
- Integration of photoperiodic and temperature cues by the circadian clock to regulate insect seasonal adaptations.Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology · 2024Review
- Gene model for the ortholog ofmicroPublication biology · 2024Article
- Environmentally responsive reproduction: neuroendocrine signalling and the evolution of eusociality.Current opinion in insect science · 2022Review
- Treating the Onset of Diabetes Using Probiotics Along with Prebiotic from Pachyrhizus erosus in High-Fat Diet Fed Drosophila melanogaster.Probiotics and antimicrobial proteins · 2022Article
- Recovery from cold-induced reproductive dormancy is regulated by temperature-dependent AstC signaling.Current biology : CB · 2022Article
- Pool-GWAS on reproductive dormancy in Drosophila simulans suggests a polygenic architecture.G3 (Bethesda, Md.) · 2022Article
- Enhanced germline stem cell longevity in Drosophila diapause.Nature communications · 2022Article
- Natural alleles of the clock geneFrontiers in physiology · 2022Article
- Endocrine Regulation of Lifespan in Insect Diapause.Frontiers in physiology · 2022Review
- Maintenance of quiescent oocytes by noradrenergic signals.Nature communications · 2021Article
- Oviposition-promoting pars intercerebralis neurons showProceedings of the National Academy of Sciences of the United States of America · 2021Article
- DIlp7-Producing Neurons Regulate Insulin-Producing Cells inFrontiers in physiology · 2021Article
- Unique genetic signatures of local adaptation over space and time for diapause, an ecologically relevant complex trait, in Drosophila melanogaster.PLoS genetics · 2020Article
- EYES ABSENT and TIMELESS integrate photoperiodic and temperature cues to regulate seasonal physiology inProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Review
- A clinal polymorphism in the insulin signaling transcription factor foxo contributes to life-history adaptation in Drosophila.Evolution; international journal of organic evolution · 2019Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 3 countries.
Funding
Abstract
Diapause is an actively induced dormancy that has evolved in Metazoa to resist environmental stresses. In temperate regions, many diapausing insects overwinter at low temperatures by blocking embryonic, larval or adult development. Despite its Afro-tropical origin, Drosophila melanogaster migrated to temperate regions of Asia and Europe where females overwinter as adults by arresting gonadal development (reproductive diapause) at temperatures <13°C. Recent work in D. melanogaster has implicated the developmental hormones dILPs-2 and/or dILP3, and dILP5, homologues of vertebrate insulin/insulin-like growth factors (IGFs), in reproductive arrest. However, polymorphisms in timeless (tim) and couch potato (cpo) dramatically affect diapause inducibility and these dILP experiments could not exclude this common genetic variation contributing to the diapause phenotype. Here, we apply an extensive genetic dissection of the insulin signaling pathway which allows us to see both enhancements and reductions in egg development that are independent of tim and cpo variations. We show that a number of manipulations dramatically enhance diapause to ~100%. These include ablating, or reducing the excitability of the insulin-producing cells (IPCs) that express dILPs-2,3,5 employing the dilp2,3,5-/- triple mutant, desensitizing insulin signaling using a chico mutation, or inhibiting dILP2 and 5 in the hemolymph by over-expressing Imaginal Morphogenesis Protein-Late 2 (Imp-L2). In addition, triple mutant dilp2,3,5-/- females maintain high levels of diapause even when temperatures are raised in adulthood to 19°C. However at 22°C, these females all show egg development revealing that the effects are conditional on temperature and not a general female sterility. In contrast, over-expression of dilps-2/5 or enhancing IPC excitability, led to levels of ovarian arrest that approached zero, underscoring dILPs-2 and 5 as key antagonists of diapause.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.