ReviewCurrent opinion in structural biology2016
Cryo-EM in the study of challenging systems: the human transcription pre-initiation complex.
Review in Current opinion in structural biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Bridging technical innovation and computational advances in studies of RNA-protein assemblies.Nature reviews. Genetics · 2026Review
- SMAD7 regulates the canonical Wnt signaling through TGF-β cascade crosstalk and SMAD7/β-CATENIN transcription factor complex formation during tooth regeneration.International journal of oral science · 2026Article
- Using cryo-EM to uncover mechanisms of bacterial transcriptional regulation.Biochemical Society transactions · 2021Review
- MYC protein interactors in gene transcription and cancer.Nature reviews. Cancer · 2021Review
- A dual role for H2A.Z.1 in modulating the dynamics of RNA polymerase II initiation and elongation.Nature structural & molecular biology · 2021Article
- Recent insights into the structure of TFIID, its assembly, and its binding to core promoter.Current opinion in structural biology · 2020Review
- Long non-coding subgenomic flavivirus RNAs have extended 3D structures and are flexible in solution.EMBO reports · 2019Article
- The hRPC62 subunit of human RNA polymerase III displays helicase activity.Nucleic acids research · 2019Article
- Methods for merging data sets in electron cryo-microscopy.Acta crystallographica. Section D, Structural biology · 2019Article
- Structural basis of DNA lesion recognition for eukaryotic transcription-coupled nucleotide excision repair.DNA repair · 2018Review
- Towards a mechanistic understanding of core promoter recognition from cryo-EM studies of human TFIID.Current opinion in structural biology · 2017Review
- Cryo-EM: beyond the microscope.Current opinion in structural biology · 2017Review
- Paused RNA polymerase II inhibits new transcriptional initiation.Nature genetics · 2017Article
- Structural Insights into the Eukaryotic Transcription Initiation Machinery.Annual review of biophysics · 2017Review
- Membrane Fusion Involved in Neurotransmission: Glimpse from Electron Microscope and Molecular Simulation.Frontiers in molecular neuroscience · 2017Review
- Structural dynamics and DNA interaction of human TFIID.Transcription · 2017Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Single particle cryo-Electron Microscopy (cryo-EM) is a technique that allows the structural characterization of macromolecules without the need for crystallization. For certain type of samples that are ideally suited for cryo-EM studies it has been possible to reach high-resolution structures following relatively standard procedures. Other biological systems remain highly challenging, even for cryo-EM. Challenges may involve the scarcity of the sample, poor stability of the complexes, and most often, the intrinsic flexibility of biological molecules. Among these challenging samples are large eukaryotic transcription complexes, which suffer from all such shortcomings. Here we report how we have recently tried to overcome those challenges in order to improve our structural understanding of the human transcription pre-initiation complex assembly and the transcription initiation process. Parallel efforts have also been carried out for budding yeast transcription initiation complexes, allowing comparisons that establish both the overall conservation and the specific structural differences between the two systems.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.