ArticlePlant physiology2016
Class II Cytochrome P450 Reductase Governs the Biosynthesis of Alkaloids.
Article in Plant physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 50 citations in OpenAlex.
- A yeast-based platform for etoposide production via yatein bioconversion.Metabolic engineering communications · 2026Article
- Enzymatic and Biophysical Analysis of two Highly Related Cytochrome P450 Reductases frombioRxiv : the preprint server for biology · 2026Article
- Unraveling the Role of P450 Reductase in Herbicide Metabolic Resistance Mechanism.Plant direct · 2025Article
- Genome-based discovery of pachysiphine synthases in Tabernaemontana elegans.The Plant journal : for cell and molecular biology · 2024Article
- Heterologous production of corosolic acid, a phyto-insulin, in agroinfiltratedPlant biotechnology (Tokyo, Japan) · 2024Article
- Cloning and Functional Characterization of NADPH-Cytochrome P450 Reductases inMolecules (Basel, Switzerland) · 2023Article
- Suppression of pyrrolidine ring biosynthesis and its effects on gene expression and subsequent accumulation of anatabine in leaves of tobacco (N. tabacum L.).BMC genomics · 2023Article
- Biochemical Alterations in Triticale Seedlings Pretreated with Selective Herbicide and Subjected to Drought or Waterlogging Stress.Plants (Basel, Switzerland) · 2023Article
- Class I and II NADPH-cytochrome P450 reductases exhibit different roles in triterpenoid biosynthesis inFrontiers in plant science · 2023Article
- The Vinca minor genome highlights conserved evolutionary traits in monoterpene indole alkaloid synthesis.G3 (Bethesda, Md.) · 2022Article
- De novo transcriptome assembly of Conium maculatum L. to identify candidate genes for coniine biosynthesis.Scientific reports · 2022Article
- In vivo characterization of key iridoid biosynthesis pathway genes in catnip (Nepeta cataria).Planta · 2022Article
- RNA In Situ Hybridization of Paraffin Sections to Characterize the Multicellular Compartmentation of Plant Secondary Metabolisms.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Discovery and Characterization of Oxidative Enzymes Involved in Monoterpenoid Indole Alkaloid Biosynthesis.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Membrane anchoring facilitates colocalization of enzymes in plant cytochrome P450 redox systems.Communications biology · 2021Article
- Optimization of Tabersonine Methoxylation to Increase Vindoline Precursor Synthesis in Yeast Cell Factories.Molecules (Basel, Switzerland) · 2021Article
- Diversity in Chemical Structures and Biological Properties of Plant Alkaloids.Molecules (Basel, Switzerland) · 2021Review
- Alternative splicing creates a pseudo-strictosidine β-d-glucosidase modulating alkaloid synthesis in Catharanthus roseus.Plant physiology · 2021Article
- Comparative Analysis of NADPH-Cytochrome P450 Reductases From Legumes for Heterologous Production of Triterpenoids in TransgenicFrontiers in plant science · 2021Article
- Identifying Genes Involved in alkaloid Biosynthesis inBiomolecules · 2020Article
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Authors and funding
19 authors at 2 institutions in 3 countries.
Funding
Abstract
Expansion of the biosynthesis of plant specialized metabolites notably results from the massive recruitment of cytochrome P450s that catalyze multiple types of conversion of biosynthetic intermediates. For catalysis, P450s require a two-electron transfer catalyzed by shared cytochrome P450 oxidoreductases (CPRs), making these auxiliary proteins an essential component of specialized metabolism. CPR isoforms usually group into two distinct classes with different proposed roles, namely involvement in primary and basal specialized metabolisms for class I and inducible specialized metabolism for class II. By studying the role of CPRs in the biosynthesis of monoterpene indole alkaloids, we provide compelling evidence of an operational specialization of CPR isoforms in Catharanthus roseus (Madagascar periwinkle). Global analyses of gene expression correlation combined with transcript localization in specific leaf tissues and gene-silencing experiments of both classes of CPR all point to the strict requirement of class II CPRs for monoterpene indole alkaloid biosynthesis with a minimal or null role of class I. Direct assays of interaction and reduction of P450s in vitro, however, showed that both classes of CPR performed equally well. Such high specialization of class II CPRs in planta highlights the evolutionary strategy that ensures an efficient reduction of P450s in specialized metabolism.
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