ArticleGenes & development2016
Functional interplay between Mediator and TFIIB in preinitiation complex assembly in relation to promoter architecture.
Article in Genes & development, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 40 citations in OpenAlex.
- The Mediator complex: co-transcriptional regulation and beyond.RNA biology · 2026Review
- Analysis of host and viral nascent and steady-state RNA levels in a human neuronal model of herpes simplex virus 1 infection.PLoS pathogens · 2026Article
- Unique Role of Med8 in Ace2 Recruitment and Target Gene Expression inJournal of microbiology and biotechnology · 2025Article
- From Genotype to Phenotype: How Enhancers Control Gene Expression and Cell Identity in Hematopoiesis.HemaSphere · 2023Review
- Regulation of the RNA polymerase II pre-initiation complex by its associated coactivators.Nature reviews. Genetics · 2023Review
- Yeast Mediator facilitates transcription initiation at most promoters via a Tail-independent mechanism.Molecular cell · 2022Article
- The Mediator Complex: A Central Coordinator of Plant Adaptive Responses to Environmental Stresses.International journal of molecular sciences · 2022Review
- Architectural Mediator subunits are differentially essential for global transcription in Saccharomyces cerevisiae.Genetics · 2021Article
- A Role for the Mre11-Rad50-Xrs2 Complex in Gene Expression and Chromosome Organization.Molecular cell · 2021Article
- PPARβ/δ recruits NCOR and regulates transcription reinitiation of ANGPTL4.Nucleic acids research · 2019Article
- Article
- Twenty years of Mediator complex structural studies.Biochemical Society transactions · 2019Review
- Phase Separation and Transcription Regulation: Are Super-Enhancers and Locus Control Regions Primary Sites of Transcription Complex Assembly?BioEssays : news and reviews in molecular, cellular and developmental biology · 2019Review
- Mediator Is Essential for Small Nuclear and Nucleolar RNA Transcription in Yeast.Molecular and cellular biology · 2018Article
- Article
- Eukaryotic core promoters and the functional basis of transcription initiation.Nature reviews. Molecular cell biology · 2018Review
- Transcription regulation by the Mediator complex.Nature reviews. Molecular cell biology · 2018Review
- Genome-wide characterization of Mediator recruitment, function, and regulation.Transcription · 2017Review
- Article
- Toward understanding of the mechanisms of Mediator function in vivo: Focus on the preinitiation complex assembly.Transcription · 2017Review
Corrections and comments
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Authors and funding
12 authors at 6 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mediator is a large coregulator complex conserved from yeast to humans and involved in many human diseases, including cancers. Together with general transcription factors, it stimulates preinitiation complex (PIC) formation and activates RNA polymerase II (Pol II) transcription. In this study, we analyzed how Mediator acts in PIC assembly using in vivo, in vitro, and in silico approaches. We revealed an essential function of the Mediator middle module exerted through its Med10 subunit, implicating a key interaction between Mediator and TFIIB. We showed that this Mediator-TFIIB link has a global role on PIC assembly genome-wide. Moreover, the amplitude of Mediator's effect on PIC formation is gene-dependent and is related to the promoter architecture in terms of TATA elements, nucleosome occupancy, and dynamics. This study thus provides mechanistic insights into the coordinated function of Mediator and TFIIB in PIC assembly in different chromatin contexts.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.