ArticleApplied microbiology and biotechnology2016
Adhesion of the genome-sequenced Lactococcus lactis subsp. cremoris IBB477 strain is mediated by specific molecular determinants.
Article in Applied microbiology and biotechnology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 22 citations in OpenAlex.
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- Screening for probiotic properties and potential immunogenic effects of lactobacilli strains isolated from various food products.Frontiers in microbiology · 2024Article
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- Probiotic Gastrointestinal Transit and Colonization After Oral Administration: A Long Journey.Frontiers in cellular and infection microbiology · 2021Review
- Impact of Prebiotics and Synbiotics AdministeredAnimals : an open access journal from MDPI · 2020Article
- GlaR (YugA)-a novel RpiR-family transcription activator of the Leloir pathway of galactose utilization in Lactococcus lactis IL1403.MicrobiologyOpen · 2019Article
- Comparative proteomic analysis of four biotechnological strains Lactococcus lactis through label-free quantitative proteomics.Microbial biotechnology · 2019Article
- Molecular, physiological and phylogenetic traits of Lactococcus 936-type phages from distinct dairy environments.Scientific reports · 2018Article
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- Surface proteins involved in the adhesion of Streptococcus salivarius to human intestinal epithelial cells.Applied microbiology and biotechnology · 2018Article
- Characterization of Mucus-Related Properties ofFrontiers in physiology · 2018Article
- Toxicity of Food-Grade TiOFrontiers in microbiology · 2018Article
- In vitro characteristics of Lactobacillus spp. strains isolated from the chicken digestive tract and their role in the inhibition of Campylobacter colonization.MicrobiologyOpen · 2017Article
- Lactic acid bacteria - promising vaccine vectors: possibilities, limitations, doubts.Journal of applied microbiology · 2017Review
- Contribution of plasmid-encoded peptidase S8 (PrtP) to adhesion and transit in the gut of Lactococcus lactis IBB477 strain.Applied microbiology and biotechnology · 2017Article
- Surface Proteins ofFrontiers in microbiology · 2017Review
- Functional Characterization of NovelFrontiers in microbiology · 2017Article
- Article
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Understanding the nature of mucus-microbe interactions will provide important information that can help to elucidate the mechanisms underlying probiotic adhesion. This study focused on the adhesive properties of the Lactococcus lactis subsp. cremoris IBB477 strain, previously shown to persist in the gastrointestinal tract of germ-free rats. The shear flow-induced detachment of L. lactis cells was investigated under laminar flow conditions. Such a dynamic approach demonstrated increased adhesion to bare and mucin-coated polystyrene for IBB477, compared to that observed for the MG1820 control strain. To identify potential genetic determinants giving adhesive properties to IBB477, the improved high-quality draft genome sequence comprising chromosome and five plasmids was obtained and analysed. The number of putative adhesion proteins was determined on the basis of surface/extracellular localisation and/or the presence of adhesion domains. To identify proteins essential for the IBB477 specific adhesion property, nine deletion mutants in chromosomal genes have been constructed and analysed using adhesion tests on bare polystyrene as well as mucin-, fibronectin- or collagen IV-coated polystyrene plates in comparison to the wild-type strain. These experiments demonstrated that gene AJ89_07570 encoding a protein containing DUF285, MucBP and four Big_3 domains is involved in adhesion to bare and mucin-coated polystyrene. To summarise, in the present work, we characterised the adhesion of IBB477 under laminar flow conditions; identified the putative adherence factors present in IBB477, which is the first L. lactis strain exhibiting adhesive and mucoadhesive properties to be sequenced and demonstrated that one of the proteins containing adhesion domains contributes to adhesion.
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