ArticleBMC genomics2016
Single-cell RNA sequencing reveals dynamic changes in A-to-I RNA editome during early human embryogenesis.
Article in BMC genomics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 35 citations in OpenAlex.
- RNA modifications in health and disease: from mechanistic insights to therapeutic applications.Precision clinical medicine · 2025Review
- Epitranscriptome-wide profiling identifies RNA editing events regulated by ADAR1 that are associated with DNA repair mechanisms in human TK6 cells.Frontiers in genetics · 2025Article
- Review
- Global A-to-I RNA editing during myogenic differentiation of goat MuSCs.Frontiers in veterinary science · 2024Article
- RBPs: an RNA editor's choice.Frontiers in molecular biosciences · 2024Review
- Identification and Interpretation of A-to-I RNA Editing Events in Insect Transcriptomes.International journal of molecular sciences · 2023Review
- Profiling A-to-I RNA editing during mouse somatic reprogramming at the single-cell level.Heliyon · 2023Article
- The role of post-transcriptional modifications during development.Biologia futura · 2023Review
- Recurrent RNA edits in human preimplantation potentially enhance maternal mRNA clearance.Communications biology · 2022Article
- Granulosa Cell Specific Loss of Adar in Mice Delays Ovulation, Oocyte Maturation and Leads to Infertility.International journal of molecular sciences · 2022Article
- Global RNA editing identification and characterization during human pluripotent-to-cardiomyocyte differentiation.Molecular therapy. Nucleic acids · 2021Article
- Processing ofRNA (New York, N.Y.) · 2020Article
- Pig-specific RNA editing during early embryo development revealed by genome-wide comparisons.FEBS open bio · 2020Article
- Spatiotemporal mapping of RNA editing in the developing mouse brain using in situ sequencing reveals regional and cell-type-specific regulation.BMC biology · 2020Article
- Dynamic Alternative Splicing During Mouse Preimplantation Embryo Development.Frontiers in bioengineering and biotechnology · 2020Article
- Deciphering miRNAs' Action through miRNA Editing.International journal of molecular sciences · 2019Review
- Inosine RNA modifications are enriched at the codon wobble position in mouse oocytes and eggs†.Biology of reproduction · 2019Article
- Transcriptome Analyses Reveal Effects of Vitamin C-Treated Donor Cells on Cloned Bovine Embryo Development.International journal of molecular sciences · 2019Article
- Accurate identification of RNA editing sites from primitive sequence with deep neural networks.Scientific reports · 2018Article
- Antibodies specific for nucleic acid modifications.RNA biology · 2017Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundA-to-I RNA-editing mediated by ADAR (adenosine deaminase acting on RNA) enzymes that converts adenosine to inosine in RNA sequence can generate mutations and alter gene regulation in metazoans. Previous studies have shown that A-to-I RNA-editing plays vital roles in mouse embryogenesis. However, the RNA-editing activities in early human embryonic development have not been investigated.
resultsHere, we characterized genome-wide A-to-I RNA-editing activities during human early embryogenesis by profiling 68 single cells from 29 human embryos spanning from oocyte to morula stages. We demonstrate dynamic changes in genome-wide RNA-editing during early human embryogenesis in a stage-specific fashion. In parallel with ADAR expression level changes, the genome-wide A-to-I RNA-editing levels in cells remained relatively stable until 4-cell stage, but dramatically decreased at 8-cell stage, continually decreased at morula stage. We detected 37 non-synonymously RNA-edited genes, of which 5 were frequently found in cells of multiple embryonic stages. Moreover, we found that A-to-I editings in miRNA-targeted regions of a substantial number of genes preferably occurred in one or two sequential stages.
conclusionsOur single-cell analysis reveals dynamic changes in genome-wide RNA-editing during early human embryogenesis in a stage-specific fashion, and provides important insights into early human embryogenesis.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.