ArticleMolecular biology reports2017
Evaluating genomic DNA extraction methods from human whole blood using endpoint and real-time PCR assays.
Article in Molecular biology reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 74 citations in OpenAlex.
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- Genetic variation in salt taste receptors impact salt intake and blood pressure.Scientific reports · 2023Article
- Diversity of Mitochondrial DNA Haplogroups and Their Association with Bovine Antral Follicle Count.Animals : an open access journal from MDPI · 2022Article
- Prevalence, Diversity, and Risk Factors for Cervical HPV Infection in Women Screened for Cervical Cancer in Belém, Pará, Northern Brazil.Pathogens (Basel, Switzerland) · 2022Article
- Detection of Ovine or Bovine Milk Components in Commercial Camel Milk Powder Using a PCR-Based Method.Molecules (Basel, Switzerland) · 2022Article
- Mutations in exon region of BRCA1-related RING domain 1 gene and risk of breast cancer.Molecular genetics & genomic medicine · 2022Article
- Associations of galectin-3 expression and LGALS-3 (rs4652) gene variant with coronary artery disease risk in diabetics.Journal of medical biochemistry · 2021Article
- Association between erythema nodosum/ erythema induratum of Bazin and Mycobacterium tuberculosis infection in Koreans.Indian journal of dermatology, venereology and leprology · 2021Article
- Aberrant Methylation of Aging-Related Genes in Asthma.Frontiers in molecular biosciences · 2021Article
- Identification of lncRNA-Protein Interactions by CLIP and RNA Pull-Down Assays.Methods in molecular biology (Clifton, N.J.) · 2021Article
- The Utilization of Linear Polylysine Coupled with Mechanic Forces to Extract Microbial DNA from Different Matrices.Microorganisms · 2020Article
- Parallel DNA Extraction From Whole Blood for Rapid Sample Generation in Genetic Epidemiological Studies.Frontiers in genetics · 2020Article
- Variable DNA methylation of aging-related genes is associated with male COPD.Respiratory research · 2019Article
- Measuring coverage and accuracy of whole-exome sequencing in clinical context.Genetics in medicine : official journal of the American College of Medical Genetics · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The extraction of genomic DNA is the crucial first step in large-scale epidemiological studies. Though there are many popular DNA isolation methods from human whole blood, only a few reports have compared their efficiencies using both end-point and real-time PCR assays. Genomic DNA was extracted from coronary artery disease patients using solution-based conventional protocols such as the phenol-chloroform/proteinase-K method and a non-phenolic non-enzymatic Rapid-Method, which were evaluated and compared vis-a-vis a commercially available silica column-based Blood DNA isolation kit. The appropriate method for efficiently extracting relatively pure DNA was assessed based on the total DNA yield, concentration, purity ratios (A
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.