ArticlePloS one
Multiplexed Molecular Imaging of Biomarker-Targeted SERS Nanoparticles on Fresh Tissue Specimens with Channel-Compressed Spectrometry.
Article in PloS one. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 39 citations in OpenAlex.
- Surface-Enhanced Raman Spectroscopy in Breast Cancer Detection: A Bibliometric Review and Landscape of Global Trends.Biosensors · 2026Review
- Innovative optical imaging strategies for monitoring immunotherapy in the tumor microenvironments.Cancer medicine · 2024Review
- Dual probe difference specimen imaging for prostate cancer margin assessment.Journal of biomedical optics · 2023Article
- Design and Synthesis of SERS Materials for In Vivo Molecular Imaging and Biosensing.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Review
- PLGA-Gold Nanocomposite: Preparation and Biomedical Applications.Pharmaceutics · 2022Review
- SERS Tags for Biomedical Detection and Bioimaging.Theranostics · 2022Review
- Multiplexed Raman Imaging in Tissues and Living Organisms.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Plasmonic nanobiosensors for detection of microRNA cancer biomarkers in clinical samples.The Analyst · 2020Article
- Image-guided tumor surgery: The emerging role of nanotechnology.Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology · 2020Review
- Surface-Enhanced Raman Spectroscopy for Cancer Immunotherapy Applications: Opportunities, Challenges, and Current Progress in Nanomaterial Strategies.Nanomaterials (Basel, Switzerland) · 2020Review
- Topical dual-probe staining using quantum dot-labeled antibodies for identifying tumor biomarkers in fresh specimens.PloS one · 2020Article
- Contemporary Approach to Locally Advanced Oral Cavity Squamous Cell Carcinoma.Current oncology reports · 2019Review
- Effect of staining temperature on topical dual stain imaging of tissue specimens for tumor identification.Proceedings of SPIE--the International Society for Optical Engineering · 2019Article
- Emerging Intraoperative Imaging Modalities to Improve Surgical Precision.Molecular imaging and biology · 2018Review
- Ratiometric optical nanoprobes enable accurate molecular detection and imaging.Chemical Society reviews · 2018Review
- Microscopic investigation of" topically applied nanoparticles for molecular imaging of fresh tissue surfaces.Journal of biophotonics · 2018Article
- Common Aspects Influencing the Translocation of SERS to Biomedicine.Current medicinal chemistry · 2018Review
- Article
- Raman-Encoded Molecular Imaging with Topically Applied SERS Nanoparticles for Intraoperative Guidance of Lumpectomy.Cancer research · 2017Article
- Optimizing fresh specimen staining for rapid identification of tumor biomarkers during surgery.Theranostics · 2017Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Biomarker-targeted surface-enhanced Raman scattering (SERS) nanoparticles (NPs) have been explored as a viable option for targeting and imaging multiple cell-surface protein biomarkers of cancer. While it has been demonstrated that this Raman-encoded molecular imaging (REMI) technology may potentially be used to guide tumor-resection procedures, the REMI strategy would benefit from further improvements in imaging speed. Previous implementations of REMI have utilized 1024 spectral channels (camera pixels) in a commercial spectroscopic CCD to detect the spectral signals from multiplexed SERS NPs, a strategy that enables accurate demultiplexing of the relative concentration of each NP "flavor" within a mixture. Here, we investigate the ability to significantly reduce the number of spectral-collection channels while maintaining accurate imaging and demultiplexing of up to five SERS NP flavors, a strategy that offers the potential for improved imaging speed and/or detection sensitivity in future systems. This strategy was optimized by analyzing the linearity of five multiplexed flavors of SERS NPs topically applied on tissues. The accuracy of this binning approach was then validated by staining tumor xenografts and human breast tumor specimens with a mixture of five NP flavors (four targeted NPs and one untargeted NP) and performing ratiometric imaging of specific vs. nonspecific NP accumulation. We demonstrate that with channel-compressed spectrometry using as few as 16 channels, it is possible to perform REMI with five NP flavors, with < 20% error, at low concentrations (< 10 pM) that are relevant for clinical applications.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.