ArticlePloS one
Long Term Ex Vivo Culture and Live Imaging of Drosophila Larval Imaginal Discs.
Article in PloS one. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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Who cites it
24 citing papers in PubMed, 42 citations in OpenAlex.
- NADThe EMBO journal · 2026Article
- Bellymount-pulsed tracking: a novel approach for real-time in vivo imaging of Drosophila abdominal tissues.G3 (Bethesda, Md.) · 2025Article
- Calcium Imaging in Drosophila.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Bellymount-Pulsed Tracking: A Novel Approach for Real-Time In vivo Imaging ofbioRxiv : the preprint server for biology · 2024Article
- Kinetics of blood cell differentiation during hematopoiesis revealed by quantitative long-term live imaging.eLife · 2023Article
- Patterning of theFrontiers in cell and developmental biology · 2023Review
- Article
- An improved organ explant culture method reveals stem cell lineage dynamics in the adulteLife · 2022Article
- Article
- Cultivation and Live Imaging of Drosophila Imaginal Discs.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Novel function ofProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Early lineage segregation of the retinal basal glia in the Drosophila eye disc.Scientific reports · 2020Article
- Modulating effect of vitamin D3 on the mutagenicity and carcinogenicity of doxorubicin in Drosophila melanogaster and in silico studies.Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association · 2020Article
- PhotoGal4: A Versatile Light-Dependent Switch for Spatiotemporal Control of Gene Expression in Drosophila Explants.iScience · 2020Article
- An Immobilization Technique for Long-Term Time-Lapse Imaging of ExplantedFrontiers in cell and developmental biology · 2020Article
- Article
- Expressional Profiling of Carpet Glia in the DevelopingFrontiers in neuroscience · 2019Article
- Temporal and spatial order of photoreceptor and glia projections into optic lobe in Drosophila.Scientific reports · 2018Article
- Diverse populations of local interneurons integrate into the Drosophila adult olfactory circuit.Nature communications · 2018Article
- Ex Vivo Calcium Imaging for Visualizing Brain Responses to Endocrine Signaling in Drosophila.Journal of visualized experiments : JoVE · 2018Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Continuous imaging of live tissues provides clear temporal sequence of biological events. The Drosophila imaginal discs have been popular experimental subjects for the study of a wide variety of biological phenomena, but long term culture that allows normal development has not been satisfactory. Here we report a culture method that can sustain normal development for 18 hours and allows live imaging. The method is validated in multiple discs and for cell proliferation, differentiation and migration. However, it does not support disc growth and cannot support cell proliferation for more than 7 to 12 hr. We monitored the cellular behavior of retinal basal glia in the developing eye disc and found that distinct glia type has distinct properties of proliferation and migration. The live imaging provided direct proof that wrapping glia differentiated from existing glia after migrating to the anterior front, and unexpectedly found that they undergo endoreplication before wrapping axons, and their nuclei migrate up and down along the axons. UV-induced specific labeling of a single carpet glia also showed that the two carpet glia membrane do not overlap and suggests a tiling or repulsion mechanism between the two cells. These findings demonstrated the usefulness of an ex vivo culture method and live imaging.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.