ArticlePloS one
Quantitative Proteomics of Sleep-Deprived Mouse Brains Reveals Global Changes in Mitochondrial Proteins.
Article in PloS one. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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The trial behind it
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Who cites it
10 citing papers in PubMed.
- Chronic sleep restriction activates complement and coagulation cascades: a molecular link to accelerated brain aging.Npj biological timing and sleep · 2026Article
- The mitochondrial theory of sleep: an integrative framework for understanding sleep-wake regulation.Frontiers in psychiatry · 2026Review
- Multiomics reveal biomolecular shifts and ER stress in sleep-restricted women affecting NSC functions.iScience · 2025Article
- Chronic sleep curtailment expediates brain aging by activating the complement and coagulation cascades in mice.bioRxiv : the preprint server for biology · 2025Article
- Article
- Hippocampal neurons' cytosolic and membrane-bound ribosomal transcript profiles are differentially regulated by learning and subsequent sleep.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Sleep, brain vascular health and ageing.GeroScience · 2020Review
- Sleep disruption elevates oxidative stress in parvalbumin-positive cells of the rat cerebral cortex.Sleep · 2019Article
- Proteomic analysis of rat serum revealed the effects of chronic sleep deprivation on metabolic, cardiovascular and nervous system.PloS one · 2018Article
- Quick 96FASP for high throughput quantitative proteome analysis.Journal of proteomics · 2017Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sleep is a ubiquitous, tightly regulated, and evolutionarily conserved behavior observed in almost all animals. Prolonged sleep deprivation can be fatal, indicating that sleep is a physiological necessity. However, little is known about its core function. To gain insight into this mystery, we used advanced quantitative proteomics technology to survey the global changes in brain protein abundance. Aiming to gain a comprehensive profile, our proteomics workflow included filter-aided sample preparation (FASP), which increased the coverage of membrane proteins; tandem mass tag (TMT) labeling, for relative quantitation; and high resolution, high mass accuracy, high throughput mass spectrometry (MS). In total, we obtained the relative abundance ratios of 9888 proteins encoded by 6070 genes. Interestingly, we observed significant enrichment for mitochondrial proteins among the differentially expressed proteins. This finding suggests that sleep deprivation strongly affects signaling pathways that govern either energy metabolism or responses to mitochondrial stress. Additionally, the differentially-expressed proteins are enriched in pathways implicated in age-dependent neurodegenerative diseases, including Parkinson's, Huntington's, and Alzheimer's, hinting at possible connections between sleep loss, mitochondrial stress, and neurodegeneration.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.