ArticleJournal of visualized experiments : JoVE2016
Deferred Growth Inhibition Assay to Quantify the Effect of Bacteria-derived Antimicrobials on Competition.
Article in Journal of visualized experiments : JoVE, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
12 citing papers in PubMed, 1 synthesis or guideline pooled it.
- From Microbial Dynamics to Functionality in the Rhizosphere: A Systematic Review of the Opportunities With Synthetic Microbial Communities.Frontiers in plant science · 2021Pooled it
- A Complete Genome Resource and Bio-Control Activity of Soil-Isolated Pseudomonas citronellolis Strain M03 Against Onion-Pathogenic Burkholderia Species.MicrobiologyOpen · 2026Article
- Isolation of Halophilic and Halotolerant Bacterial Strains, Screening for Bioactive Compounds and Characterisation of Metabolites Produced by Pseudoalteromonas sp. ASV78.Environmental microbiology reports · 2025Article
- Propagation of antibacterial cold atmospheric pressure plasma through small-bore tubing.PloS one · 2025Article
- Whole genome sequencing and characterization of Corynebacterium isolated from the healthy and dry eye ocular surface.BMC microbiology · 2024Article
- Direct Detection of Antibacterial-Producing Soil Isolates Utilizing a Novel High-Throughput Screening Assay.Microorganisms · 2022Article
- Staphylococcus aureus in Substrates for Black Soldier Fly Larvae (Hermetia illucens) and Its Dynamics during Rearing.Microbiology spectrum · 2021Article
- Article
- Cultivable microbiota associated with Aurelia aurita and Mnemiopsis leidyi.MicrobiologyOpen · 2020Article
- Evidence of Biocontrol Activity of Bioinoculants Against a Human Pathogen,Frontiers in microbiology · 2020Article
- Development of a Robust and Quantitative High-Throughput Screening Method for Antibiotic Production in Bacterial Libraries.ACS omega · 2019Article
- A prostate derived commensal Staphylococcus epidermidis strain prevents and ameliorates induction of chronic prostatitis by UPEC infection.Scientific reports · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
Competitive exclusion can occur in microbial communities when, for example, an inhibitor-producing strain outcompetes its competitor for an essential nutrient or produces antimicrobial compounds that its competitor is not resistant to. Here we describe a deferred growth inhibition assay, a method for assessing the ability of one bacterium to inhibit the growth of another through the production of antimicrobial compounds or through competition for nutrients. This technique has been used to investigate the correlation of nasal isolates with the exclusion of particular species from a community. This technique can also be used to screen for lantibiotic producers or potentially novel antimicrobials. The assay is performed by first culturing the test inhibitor-producing strain overnight on an agar plate, then spraying over the test competitor strain and incubating again. After incubation, the extent of inhibition can be measured quantitatively, through the size of the zone of clearing around the inhibitor-producing strain, and qualitatively, by assessing the clarity of the inhibition zone. Here we present the protocol for the deferred inhibition assay, describe ways to minimize variation between experiments, and define a clarity scale that can be used to qualitatively assess the degree of inhibition.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.