ArticleAnalytical chemistry2016
Adductomics Pipeline for Untargeted Analysis of Modifications to Cys34 of Human Serum Albumin.
Article in Analytical chemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed.
- Use of Human Serum Albumin CysAntioxidants (Basel, Switzerland) · 2026Review
- Adductomics of Newborn Dried Blood Spots Detects Constituents of Maternal Smoking During Pregnancy and Associated Oxidative Stress Exposure.Antioxidants (Basel, Switzerland) · 2026Article
- A sensitive method for simultaneous screening exposure to 14 alkylating agents based on tripeptide adducts: an efficient approach for chemical weapon verification and chemical impurity profiling in plasma samples.Analytical and bioanalytical chemistry · 2025Article
- HSA Adductomics in the Shanghai Women's Health Study Links Lung Cancer in Never-Smokers with Air Pollution, Redox Biology, and One-Carbon Metabolism.Antioxidants (Basel, Switzerland) · 2025Article
- Multi-adductomics: Advancing mass spectrometry techniques for comprehensive exposome characterization.Trends in analytical chemistry : TRAC · 2024Article
- Cord Blood Adductomics Reveals Oxidative Stress Exposure Pathways of Bronchopulmonary Dysplasia.Antioxidants (Basel, Switzerland) · 2024Article
- Old poisons, new signaling molecules: the case of hydrogen cyanide.Journal of experimental botany · 2023Article
- HSA Adductomics Reveals Sex Differences in NHL Incidence and Possible Involvement of Microbial Translocation.Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology · 2023Article
- A nested case-control study of untargeted albumin adductomics and acute myeloid leukemia.International journal of cancer · 2023Article
- Overview of Adductomics in Toxicology.Current protocols · 2023Article
- Pathways to Identify ElectrophilesChemical research in toxicology · 2022Article
- Exposure to diesel engine exhaust and alterations to the Cys34/Lys525 adductome of human serum albumin.Environmental toxicology and pharmacology · 2022Article
- A proteomics strategy for the identification of multiple sites in sulfur mustard-modified HSA and screening potential biomarkers for retrospective analysis of exposed human plasma.Analytical and bioanalytical chemistry · 2022Article
- Quo vadis blood protein adductomics?Archives of toxicology · 2022Review
- Applications of Adductomics in Chemically Induced Adverse Outcomes and Major Emphasis on DNA Adductomics: A Pathbreaking Tool in Biomedical Research.International journal of molecular sciences · 2021Review
- Utilizing a Biology-Driven Approach to Map the Exposome in Health and Disease: An Essential Investment to Drive the Next Generation of Environmental Discovery.Environmental health perspectives · 2021Article
- Novel 4-Hydroxybenzyl Adducts in Human Hemoglobin: Structures and Mechanisms of Formation.Chemical research in toxicology · 2021Article
- Metabolism and biomarkers of heterocyclic aromatic amines in humans.Genes and environment : the official journal of the Japanese Environmental Mutagen Society · 2021Review
- Biomonitoring of Ambient Outdoor Air Pollutant Exposure in Humans Using Targeted Serum Albumin Adductomics.Chemical research in toxicology · 2021Article
- A Workflow towards the Reproducible Identification and Quantitation of Protein Carbonylation Sites in Human Plasma.Antioxidants (Basel, Switzerland) · 2021Article
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8 authors.
Funding
Abstract
An important but understudied class of human exposures is comprised of reactive electrophiles that cannot be measured in vivo because they are short-lived. An avenue for assessing these meaningful exposures focuses on adducts from reactions with nucleophilic loci of blood proteins, particularly Cys34 of human serum albumin, which is the dominant scavenger of reactive electrophiles in serum. We developed an untargeted analytical scheme and bioinformatics pipeline for detecting, quantitating, and annotating Cys34 adducts in tryptic digests of human serum/plasma. The pipeline interrogates tandem mass spectra to find signatures of the Cys34-containing peptide, obtains accurate masses of putative adducts, quantitates adduct levels relative to a "housekeeping peptide", and annotates modifications based on a combination of retention time, accurate mass, elemental composition, and database searches. We used the adductomics pipeline to characterize 43 adduct features in archived plasma from healthy human subjects and found several that were highly associated with smoking status, race, and other covariates. Since smoking is a strong risk factor for cancer and cardiovascular disease, our ability to discover adducts that distinguish smokers from nonsmokers with untargeted adductomics indicates that the pipeline is suitable for use in epidemiologic studies. In fact, adduct features were both positively and negatively associated with smoking, indicating that some adducts arise from reactions between Cys34 and constituents of cigarette smoke (e.g., ethylene oxide and acrylonitrile) while others (Cys34 oxidation products and disulfides) appear to reflect alterations in the serum redox state that resulted in reduced adduct levels in smokers.
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