ArticleeLife2016
Quantitative imaging reveals real-time Pou5f3-Nanog complexes driving dorsoventral mesendoderm patterning in zebrafish.
Article in eLife, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 43 citations in OpenAlex.
- Current capabilities and future perspectives of FCS: super-resolution microscopy, machine learning, and in vivo applications.Communications biology · 2023Review
- Maternal mRNA deadenylation and allocation via Rbm14 condensates facilitate vertebrate blastula development.The EMBO journal · 2023Article
- Review
- Review
- Apelin, APJ, and ELABELA: Role in Placental Function, Pregnancy, and Foetal Development-An Overview.Cells · 2021Review
- The Role of Apelin/Apelin Receptor in Energy Metabolism and Water Homeostasis: A Comprehensive Narrative Review.Frontiers in physiology · 2021Review
- Nanog safeguards early embryogenesis against global activation of maternal β-catenin activity by interfering with TCF factors.PLoS biology · 2020Article
- Emerging roles of APLN and APELA in the physiology and pathology of the female reproductive system.PeerJ · 2020Article
- Control of Hox transcription factor concentration and cell-to-cell variability by an auto-regulatory switch.Development (Cambridge, England) · 2019Article
- Regulation of zebrafish dorsoventral patterning by phase separation of RNA-binding protein Rbm14.Cell discovery · 2019Article
- Biological functions of Elabela, a novel endogenous ligand of APJ receptor.Journal of cellular physiology · 2018Review
- Article
- Fluorescence techniques in developmental biology.Journal of biosciences · 2018Review
- The primary role of zebrafishDevelopment (Cambridge, England) · 2018Article
- Determination of diffusion coefficients in live cells using fluorescence recovery after photobleaching with wide-field fluorescence microscopy.Biophysics and physicobiology · 2018Article
- Alternative Progenitor Cells Compensate to Rebuild the Coronary Vasculature in Elabela- and Apj-Deficient Hearts.Developmental cell · 2017Article
- The rise of photoresponsive protein technologies applicationsF1000Research · 2017Review
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Formation of the three embryonic germ layers is a fundamental developmental process that initiates differentiation. How the zebrafish pluripotency factor Pou5f3 (homologous to mammalian Oct4) drives lineage commitment is unclear. Here, we introduce fluorescence lifetime imaging microscopy and fluorescence correlation spectroscopy to assess the formation of Pou5f3 complexes with other transcription factors in real-time in gastrulating zebrafish embryos. We show, at single-cell resolution in vivo, that Pou5f3 complexes with Nanog to pattern mesendoderm differentiation at the blastula stage. Later, during gastrulation, Sox32 restricts Pou5f3-Nanog complexes to the ventrolateral mesendoderm by binding Pou5f3 or Nanog in prospective dorsal endoderm. In the ventrolateral endoderm, the Elabela / Aplnr pathway limits Sox32 levels, allowing the formation of Pou5f3-Nanog complexes and the activation of downstream BMP signaling. This quantitative model shows that a balance in the spatiotemporal distribution of Pou5f3-Nanog complexes, modulated by Sox32, regulates mesendoderm specification along the dorsoventral axis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.