ArticleMolecular biology of the cell2016
Neurite outgrowth is driven by actin polymerization even in the presence of actin polymerization inhibitors.
Article in Molecular biology of the cell, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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Who cites it
26 citing papers in PubMed, 47 citations in OpenAlex.
- Laminin-triggered integrin signaling potentiates γ-enolase-induced neurite outgrowth.Molecular and cellular biochemistry · 2026Article
- Dual-stiffness nanoparticles for compartment-specific drug delivery in stroke.Nature communications · 2026Article
- Actin depolymerization promotes axon regeneration by restoring axonal mitochondrial transport in mouse models of optic neuropathy.Science translational medicine · 2026Article
- Cortical tension as a mechanical barrier to safeguard against premature differentiation during neurogenesis.Research square · 2025Article
- Cortical tension as a mechanical barrier to safeguard against premature differentiation during neurogenesis.bioRxiv : the preprint server for biology · 2025Article
- A critical role for the fascin family of actin bundling proteins in axon development, brain wiring and function.Molecular and cellular neurosciences · 2025Article
- Rho kinase isoforms in neurodegeneration: from cellular functions to therapeutic targets.Molecular biology reports · 2025Review
- Thymosin β4 promotes zebrafish Mauthner axon regeneration by facilitating actin polymerization through binding to G-actin.BMC biology · 2024Article
- Review
- Actin polymerization and depolymerization in developing vertebrates.Frontiers in physiology · 2023Review
- Towards a comprehensive approach for characterizing cell activity in bright-field microscopic images.Scientific reports · 2022Article
- Unique Actions of GABA Arising from Cytoplasmic Chloride Microdomains.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Article
- Membrane Cholesterol Is a Critical Determinant for Hippocampal Neuronal Polarity.Frontiers in molecular neuroscience · 2021Article
- Article
- Branched actin networks are assembled on microtubules by adenomatous polyposis coli for targeted membrane protrusion.The Journal of cell biology · 2020Article
- Mechanisms of Axon Elongation Following CNS Injury: What Is Happening at the Axon Tip?Frontiers in cellular neuroscience · 2020Article
- Radial somatic F-actin organization affects growth cone dynamics during early neuronal development.EMBO reports · 2019Article
- Cytolinker Gas2L1 regulates axon morphology through microtubule-modulated actin stabilization.EMBO reports · 2019Article
- Actin-microtubule crosstalk in cell biology.Nature reviews. Molecular cell biology · 2019Review
- The ubiquitin ligase PHR promotes directional regrowth of spinal zebrafish axons.Communications biology · 2019Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
Actin polymerization is a universal mechanism to drive plasma membrane protrusion in motile cells. One apparent exception to this rule is continuing, or even accelerated outgrowth of neuronal processes in the presence of actin polymerization inhibitors. This fact together with a key role of microtubule dynamics in neurite outgrowth led to the concept that microtubules directly drive plasma membrane protrusion, either in the course of polymerization or motor-driven sliding. Surprisingly, a possibility that unextinguished actin polymerization drives neurite outgrowth in the presence of actin drugs was not explored. We show that cultured hippocampal neurons treated with cytochalasin D or latrunculin B contained dense accumulations of branched actin filaments at ∼50% of neurite tips at all tested drug concentrations (1-10 μM). Actin polymerization was required for neurite outgrowth, because only low concentrations of either inhibitor increased the length and/or a number of neurites, whereas high concentrations inhibited neurite outgrowth. Importantly, neurites undergoing active elongation invariably contained a bright F-actin patch at the tip, whereas actin-depleted neurites never elongated, even though they still contained dynamic microtubules. Stabilization of microtubules by taxol treatment did not stop elongation of cytochalasin d-treated neurites. We conclude that actin polymerization is indispensable for neurite elongation.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.