ArticleMolecular biology of the cell2016
Functional nanoscale coupling of Lyn kinase with IgE-FcεRI is restricted by the actin cytoskeleton in early antigen-stimulated signaling.
Article in Molecular biology of the cell, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 48 citations in OpenAlex.
- A single-molecule reporter of membrane-proximal actin detects rapid remodeling upon B cell receptor clustering.bioRxiv : the preprint server for biology · 2026Article
- Boxcar Imaging FCS Reveals Membrane Raft Stabilization Kinetics in Antigen-Stimulated Mast Cells.bioRxiv : the preprint server for biology · 2025Article
- Critical roles of plasma membrane dynamics and heterogeneity at the early stage of antigen-stimulated FcεRI signaling in mast cells.Biochemical Society transactions · 2025Review
- Antigen properties shape organization of FcεRI aggregates to tune mast cell signaling.bioRxiv : the preprint server for biology · 2025Article
- Synergy between membrane topography and domains to control signaling protein localization in mast cells facilitates their activation.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Enhancing allergy diagnosis: mass spectrometry as a complementary technique to the basophil activation test.Frontiers in allergy · 2025Review
- Lipid-driven interleaflet coupling of plasma membrane order regulates FcεRI signaling in mast cells.Biophysical journal · 2024Article
- Multiscale imaging and quantitative analysis of plasma membrane protein-cortical actin interplay.Biophysical journal · 2023Article
- Cholesterol- and actin-centered view of the plasma membrane: updating the Singer-Nicolson fluid mosaic model to commemorate its 50th anniversaryMolecular biology of the cell · 2023Article
- The plasma membrane as an adaptable fluid mosaic.Biochimica et biophysica acta. Biomembranes · 2023Review
- Multiscale imaging and quantitative analysis of plasma membrane protein-cortical actin interplay.bioRxiv : the preprint server for biology · 2023Article
- My path in the company of chemistry.Pure and applied chemistry. Chimie pure et appliquee · 2022Article
- Transbilayer Coupling of Lipids in Cells Investigated by Imaging Fluorescence Correlation Spectroscopy.The journal of physical chemistry. B · 2022Article
- Signaling Enzymes and Ion Channels Being Modulated by the Actin Cytoskeleton at the Plasma Membrane.International journal of molecular sciences · 2021Review
- Lipid-based and protein-based interactions synergize transmembrane signaling stimulated by antigen clustering of IgE receptors.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Critical Phenomena in Plasma Membrane Organization and Function.Annual review of physical chemistry · 2021Review
- Cytoskeletal Transport, Reorganization, and Fusion Regulation in Mast Cell-Stimulus Secretion Coupling.Frontiers in cell and developmental biology · 2021Review
- High-speed single-molecule imaging reveals signal transduction by induced transbilayer raft phases.The Journal of cell biology · 2020Article
- Bringing light to ER contacts and a new phase in organelle communication.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Imaging FCS delineates subtle heterogeneity in plasma membranes of resting mast cells.Molecular biology of the cell · 2020Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The allergic response is initiated on the plasma membrane of mast cells by phosphorylation of the receptor for immunoglobulin E (IgE), FcεRI, by Lyn kinase after IgE-FcεRI complexes are cross-linked by multivalent antigen. Signal transduction requires reorganization of receptors and membrane signaling proteins, but this spatial regulation is not well defined. We used fluorescence localization microscopy (FLM) and pair-correlation analysis to measure the codistribution of IgE-FcεRI and Lyn on the plasma membrane of fixed cells with 20- to 25-nm resolution. We directly visualized Lyn recruitment to IgE-FcεRI within 1 min of antigen stimulation. Parallel FLM experiments captured stimulation-induced FcεRI phosphorylation and colocalization of a saturated lipid-anchor probe derived from Lyn's membrane anchorage. We used cytochalasin and latrunculin to investigate participation of the actin cytoskeleton in regulating functional interactions of FcεRI. Inhibition of actin polymerization by these agents enhanced colocalization of IgE-FcεRI with Lyn and its saturated lipid anchor at early stimulation times, accompanied by augmented phosphorylation within FcεRI clusters. Ising model simulations provide a simplified model consistent with our results. These findings extend previous evidence that IgE-FcεRI signaling is initiated by colocalization with Lyn in ordered lipid regions and that the actin cytoskeleton regulates this functional interaction by influencing the organization of membrane lipids.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.