ReviewMicrobiology and molecular biology reviews : MMBR2016
The Blueprint of a Minimal Cell: MiniBacillus.
Review in Microbiology and molecular biology reviews : MMBR, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
33 citing papers in PubMed, 78 citations in OpenAlex.
- Post-translational modifications of GlmR integrate metabolic and stress signals to maintain cell envelope homeostasis in Bacillus subtilis.PLoS genetics · 2026Article
- Biomanufacturing Potential of Streamlined Cells.Biotechnology and bioengineering · 2025Article
- Exploring lipid diversity and minimalism to define membrane requirements for synthetic cells.FEBS letters · 2025Review
- Staying in the loop to make ends meet: roles and regulation of GlmR inbioRxiv : the preprint server for biology · 2025Article
- Design and regulation of engineered bacteria for environmental release.Nature microbiology · 2025Review
- Article
- Coenzyme A biosynthesis inmBio · 2024Article
- Metabolic Profile of the Genome-ReducedACS synthetic biology · 2024Article
- Article
- Control of asparagine homeostasis inJournal of bacteriology · 2024Article
- Bacillus subtilis, a Swiss Army Knife in Science and Biotechnology.Journal of bacteriology · 2023Review
- Greedy reduction of Bacillus subtilis genome yields emergent phenotypes of high resistance to a DNA damaging agent and low evolvability.Nucleic acids research · 2023Article
- The elements of life: A biocentric tour of the periodic table.Advances in microbial physiology · 2023Article
- How To Deal with Toxic Amino Acids: the Bipartite AzlCD Complex Exports Histidine inJournal of bacteriology · 2022Article
- The current state of SubtiWiki, the database for the model organism Bacillus subtilis.Nucleic acids research · 2022Article
- SynWiki: Functional annotation of the first artificial organism Mycoplasma mycoides JCVI-syn3A.Protein science : a publication of the Protein Society · 2022Article
- Traditional protocols and optimization methods lead to absent expression in a mycoplasma cell-free gene expression platform.Synthetic biology (Oxford, England) · 2022Article
- Redirected Stress Responses in a Genome-Minimized 'midimSystems · 2021Article
- Unchaining miniApplied and environmental microbiology · 2021Article
- Quasi-essentiality of RNase Y in Bacillus subtilis is caused by its critical role in the control of mRNA homeostasis.Nucleic acids research · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacillus subtilis is one of the best-studied organisms. Due to the broad knowledge and annotation and the well-developed genetic system, this bacterium is an excellent starting point for genome minimization with the aim of constructing a minimal cell. We have analyzed the genome of B. subtilis and selected all genes that are required to allow life in complex medium at 37°C. This selection is based on the known information on essential genes and functions as well as on gene and protein expression data and gene conservation. The list presented here includes 523 and 119 genes coding for proteins and RNAs, respectively. These proteins and RNAs are required for the basic functions of life in information processing (replication and chromosome maintenance, transcription, translation, protein folding, and secretion), metabolism, cell division, and the integrity of the minimal cell. The completeness of the selected metabolic pathways, reactions, and enzymes was verified by the development of a model of metabolism of the minimal cell. A comparison of the MiniBacillus genome to the recently reported designed minimal genome of Mycoplasma mycoides JCVI-syn3.0 indicates excellent agreement in the information-processing pathways, whereas each species has a metabolism that reflects specific evolution and adaptation. The blueprint of MiniBacillus presented here serves as the starting point for a successive reduction of the B. subtilis genome.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.