ArticleCell reports2016
DEEPN as an Approach for Batch Processing of Yeast 2-Hybrid Interactions.
Article in Cell reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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Who cites it
14 citing papers in PubMed.
- NGPINT V3: a containerized orchestration Python software for discovery of next-generation protein-protein interactions.Bioinformatics (Oxford, England) · 2025Article
- Powdery mildew effectors AVRMolecular plant pathology · 2024Article
- Dynamic Enrichment for Evaluation of Protein Networks (DEEPN): A High Throughput Yeast Two-Hybrid (Y2H) Protocol to Evaluate Networks.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Next-Generation Yeast Two-Hybrid Screening to Discover Protein-Protein Interactions.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Bioinformatic Analysis of Yeast Two-Hybrid Next-Generation Interaction Screen Data.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Interactions of ubiquitin and CHMP5 with the V domain of HD-PTP reveals role for regulation of Vps4 ATPase.Molecular biology of the cell · 2021Article
- Article
- Next-generation yeast-two-hybrid analysis with Y2H-SCORES identifies novel interactors of the MLA immune receptor.PLoS computational biology · 2021Article
- Deconvolution of Multiple Rab Binding Domains Using the Batch Yeast 2-Hybrid Method DEEPN.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Newer Methods Drive Recent Insights into Rab GTPase Biology: An Overview.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Synaptotagmin 17 controls neurite outgrowth and synaptic physiology via distinct cellular pathways.Nature communications · 2019Article
- Informatic Analysis of Sequence Data from Batch Yeast 2-Hybrid Screens.Journal of visualized experiments : JoVE · 2018Article
- A Yeast 2-Hybrid Screen in Batch to Compare Protein Interactions.Journal of visualized experiments : JoVE · 2018Article
- Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
We adapted the yeast 2-hybrid assay to simultaneously uncover multiple transient protein interactions within a single screen by using a strategy termed DEEPN (dynamic enrichment for evaluation of protein networks). This approach incorporates high-throughput DNA sequencing and computation to follow competition among a plasmid population encoding interacting partners. To demonstrate the capacity of DEEPN, we identify a wide range of ubiquitin-binding proteins, including interactors that we verify biochemically. To demonstrate the specificity of DEEPN, we show that DEEPN allows simultaneous comparison of candidate interactors across multiple bait proteins, allowing differential interactions to be identified. This feature was used to identify interactors that distinguish between GTP- and GDP-bound conformations of Rab5.
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Registered trials
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