Evidence map›Paper›PMID 27681428›Full record

ArticleCell reports2016

The Homeobox Transcription Factor RHOX10 Drives Mouse Spermatogonial Stem Cell Establishment.

Hye-Won Song, Anilkumar Bettegowda, Blue B Lake, Adrienne H Zhao, David Skarbrevik, Eric Babajanian, Meena Sukhwani, Eleen Y Shum, Mimi H Phan, Terra-Dawn M Plank and 7 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.

0numbers the graph read from it
0cells of the map it votes in
30citing papers in PubMed
11.4field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

30 citing papers in PubMed, 74 citations in OpenAlex.

  1. Article
  2. Review
  3. Epigenetic priming in the male germline.Current opinion in genetics & development · 2024
    Review
  4. Article
  5. Review
  6. Review
  7. Article
  8. Article
  9. Article
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  11. Article
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  13. Article
  14. TheProceedings of the National Academy of Sciences of the United States of America · 2021
    Article
  15. Review
  16. Article
  17. Review
  18. A single-cell view of spermatogonial stem cells.Current opinion in cell biology · 2020
    Review
  19. Review
  20. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

17 authors at 4 institutions in 2 countries.

Hye-Won SongSchool of Medicine, Department of Reproductive Medicine, University of California at San Diego, La Jolla, CA 92093, USA.
Anilkumar BettegowdaSchool of Medicine, Department of Reproductive Medicine, University of California at San Diego, La Jolla, CA 92093, USA.
Blue B LakeDepartment of Bioengineering, University of California at San Diego, La Jolla, CA 92093, USA.
Adrienne H ZhaoSchool of Medicine, Department of Reproductive Medicine, University of California at San Diego, La Jolla, CA 92093, USA.
David SkarbrevikSchool of Medicine, Department of Reproductive Medicine, University of California at San Diego, La Jolla, CA 92093, USA.
Eric BabajanianSchool of Medicine, Department of Reproductive Medicine, University of California at San Diego, La Jolla, CA 92093, USA.
Meena SukhwaniObstetrics, Gynecology, and Reproductive Sciences, University of Pittsburgh School of Medicine, Magee-Womens Research Institute, Pittsburgh, PA 15213, USA.
Eleen Y ShumSchool of Medicine, Department of Reproductive Medicine, University of California at San Diego, La Jolla, CA 92093, USA.
Mimi H PhanSchool of Medicine, Department of Reproductive Medicine, University of California at San Diego, La Jolla, CA 92093, USA.
Terra-Dawn M PlankSchool of Medicine, Department of Reproductive Medicine, University of California at San Diego, La Jolla, CA 92093, USA.
Marcy E RichardsonSchool of Medicine, Department of Reproductive Medicine, University of California at San Diego, La Jolla, CA 92093, USA.
Madhuvanthi RamaiahSchool of Medicine, Department of Reproductive Medicine, University of California at San Diego, La Jolla, CA 92093, USA.
Vaishnavi SridharSchool of Medicine, Department of Reproductive Medicine, University of California at San Diego, La Jolla, CA 92093, USA.
Dirk G de RooijReproductive Biology Group, Division of Developmental Biology, Faculty of Science, Department of Biology, Utrecht University, 3584 CH Utrecht, the Netherlands.
Kyle E OrwigObstetrics, Gynecology, and Reproductive Sciences, University of Pittsburgh School of Medicine, Magee-Womens Research Institute, Pittsburgh, PA 15213, USA.
Kun ZhangDepartment of Bioengineering, University of California at San Diego, La Jolla, CA 92093, USA.
Miles F WilkinsonSchool of Medicine, Department of Reproductive Medicine, University of California at San Diego, La Jolla, CA 92093, USA; Institute of Genomic Medicine, University of California, San Diego, La Jolla, CA 92093, USA. Electronic address: mfwilkinson@ucsd.edu.
University of California, San Diego · USMagee-Womens Research Institute · USUniversity of Pittsburgh · USUtrecht University · NL

Funding

VIRAL MALIGNANCYP30CA023100 · NCI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI DIANE M SIMEONE · 1985 to 2026
$124.9M
Spermatogonial Stem Cell Establishment and RegulationR01GM119128 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI TAN, KUN, WILKINSON, MILES FROME · 2016 to 2025
$3.6M
NCI NIH HHS P30 CA023100NIGMS NIH HHS R01 GM119128
6 · The paper itself

Abstract

The developmental origins of most adult stem cells are poorly understood. Here, we report the identification of a transcription factor-RHOX10-critical for the initial establishment of spermatogonial stem cells (SSCs). Conditional loss of the entire 33-gene X-linked homeobox gene cluster that includes Rhox10 causes progressive spermatogenic decline, a phenotype indistinguishable from that caused by loss of only Rhox10. We demonstrate that this phenotype results from dramatically reduced SSC generation. By using a battery of approaches, including single-cell-RNA sequencing (scRNA-seq) analysis, we show that Rhox10 drives SSC generation by promoting pro-spermatogonia differentiation. Rhox10 also regulates batteries of migration genes and promotes the migration of pro-spermatogonia into the SSC niche. The identification of an X-linked homeobox gene that drives the initial generation of SSCs has implications for the evolution of X-linked gene clusters and sheds light on regulatory mechanisms influencing adult stem cell generation in general.

Indexed as

Gene Expression Regulation, DevelopmentalGenes, X-LinkedAdult Germline Stem CellsAnimalsGenes, DevelopmentalHomeodomain ProteinsMaleMiceMice, KnockoutMultigene FamilyProtein IsoformsSequence Analysis, RNASingle-Cell AnalysisSpermatogenesisSpermatogoniaHomeodomain ProteinsProtein IsoformsRhox10 protein, mousegerm cellgerm line stem cellgonocyteshomeoboxprospermatogoniaRhoxspermatogenesisspermatogoniaspermatogonial stem cellstranscription factor

Identifiers

PMID27681428
PMCPMC5063083
OpenAlexW2525897934

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.