ArticleMolecular brain2016
In vivo whole-cell recording with high success rate in anaesthetized and awake mammalian brains.
Article in Molecular brain, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Associative Learning-Induced Synaptic Potentiation at the Two Major Hippocampal CA1 Inputs for Cued Memory Acquisition.Neuroscience bulletin · 2025Article
- Optogenetic Determination of Dynamic and Cell-Type-Specific Inhibitory Reversal Potentials.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2024Article
- Article
- Identifying properties of pattern completion neurons in a computational model of the visual cortex.PLoS computational biology · 2023Article
- Insulin actions on hypothalamic glucose-sensing neurones.Journal of neuroendocrinology · 2021Review
- In Vivo Whole-Cell Patch-Clamp Methods: Recent Technical Progress and Future Perspectives.Sensors (Basel, Switzerland) · 2021Review
- Compensation of physiological motion enables high-yield whole-cell recording in vivo.Journal of neuroscience methods · 2021Article
- Optimal Pipette Resistance, Seal Resistance, and Zero-Current Membrane Potential for Loose Patch or Breakthrough Whole-Cell RecordingFrontiers in neural circuits · 2020Article
- Dependence of Generation of Hippocampal CA1 Slow Oscillations on Electrical Synapses.Neuroscience bulletin · 2020Article
- NMDA receptor-gated visual responses in hippocampal CA1 neurons.The Journal of physiology · 2018Article
- Enhancement of synchronized activity between hippocampal CA1 neurons during initial storage of associative fear memory.The Journal of physiology · 2017Article
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
As a critical technique for dissection of synaptic and cellular mechanisms, whole-cell patch-clamp recording has become feasible for in vivo preparations including both anaesthetized and awake mammalian brains. However, compared with in vitro whole-cell recording, in vivo whole-cell recording often suffers from low success rates and high access resistance, preventing its wide application in physiological analysis of neural circuits. Here, we describe experimental procedures for achieving in vivo amphotericin B-perforated whole-cell recording as well as conventional (breakthrough) whole-cell recording from rats and mice. The success rate of perforated whole-cell recordings was 70-80 % in the hippocampus and neocortex, and access resistance was 40-70 MΩ. The success rate of conventional whole-cell recordings was ~50 % in the hippocampus, with access resistance of 20-40 MΩ. Recordings were stable, and in awake, head-fixed animals, ~50 % whole-cell patched neurons could be held for > 1 hr. The conventional whole-cell recording also permitted infusion of pharmacological agents, such as intracellular blockers of Na
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