ArticleBiotechnology for biofuels2016
Phenotypic characterization and comparative transcriptomics of evolved Saccharomyces cerevisiae strains with improved tolerance to lignocellulosic derived inhibitors.
Article in Biotechnology for biofuels, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Comparison of stress tolerance mechanisms between Saccharomyces cerevisiae and the multistress-tolerant Pichia kudriavzevii.FEMS yeast research · 2025Review
- Article
- Contribution ofPolish journal of microbiology · 2023Article
- How adaptive laboratory evolution can boost yeast tolerance to lignocellulosic hydrolyses.Current genetics · 2022Review
- RNA sequencing reveals metabolic and regulatory changes leading to more robust fermentation performance during short-term adaptation of Saccharomyces cerevisiae to lignocellulosic inhibitors.Biotechnology for biofuels · 2021Article
- Stress modulation as a means to improve yeasts for lignocellulose bioconversion.Applied microbiology and biotechnology · 2021Review
- Impact of Lignocellulose Pretreatment By-Products onMolecules (Basel, Switzerland) · 2021Article
- Adaptive laboratory evolution of Yarrowia lipolytica improves ferulic acid tolerance.Applied microbiology and biotechnology · 2021Article
- Multi-Faceted Systems Biology Approaches Present a Cellular Landscape of Phenolic Compound Inhibition inFrontiers in bioengineering and biotechnology · 2020Review
- Repeated cultures of3 Biotech · 2019Article
- Isolation and Characterization of the Stress-TolerantFrontiers in bioengineering and biotechnology · 2019Article
- Transcriptomic analysis of the oleaginous yeastBiotechnology for biofuels · 2019Article
- Enhanced acetic acid stress tolerance and ethanol production inBiotechnology for biofuels · 2019Article
- Saccharomyces cerevisiae strains for second-generation ethanol production: from academic exploration to industrial implementation.FEMS yeast research · 2017Review
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4 authors.
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Abstract
backgroundLignocellulosic biomass continues to be investigated as a viable source for bioethanol production. However, the pretreatment process generates inhibitory compounds that impair the growth and fermentation performance of microorganisms such as Saccharomyces cerevisiae. Pinewood specifically has been shown to be challenging in obtaining industrially relevant ethanol titers. An industrial S. cerevisiae strain was subjected to directed evolution and adaptation in pretreated pine biomass and resultant strains, GHP1 and GHP4, exhibited improved growth and fermentative ability on pretreated pine in the presence of related inhibitory compounds. A comparative transcriptomic approach was applied to identify and characterize differences in phenotypic stability of evolved strains.
resultsEvolved strains displayed different fermentative capabilities with pretreated pine that appear to be influenced by the addition or absence of 13 inhibitory compounds during pre-culturing. GHP4 performance was consistent independent of culturing conditions, while GHP1 performance was dependent on culturing with inhibitors. Comparative transcriptomics revealed 52 genes potentially associated with stress responses to multiple inhibitors simultaneously. Fluorescence microscopy revealed improved cellular integrity of both strains with mitochondria exhibiting resistance to the damaging effects of inhibitors in contrast to the parent.
conclusionsMultiple potentially novel genetic targets have been discovered for understanding stress tolerance through the characterization of our evolved strains. This study specifically examines the synergistic effects of multiple inhibitors and identified targets will guide future studies in remediating effects of inhibitors and further development of robust yeast strains for multiple industrial applications.
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