ArticleFrontiers in plant science2016
Selection of Reference Genes for Quantitative Real-Time RT-PCR Studies in Tomato Fruit of the Genotype MT-Rg1.
Article in Frontiers in plant science, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- A shade-hyposensitive tomato line shows altered auxin homeostasis and higher fruit yield under high-density field conditions.The New phytologist · 2025Article
- Conserved regions upstream ofFrontiers in plant science · 2025Article
- A stable combination of non-stable genes outperforms standard reference genes for RT-qPCR data normalization.Scientific reports · 2024Article
- Molecular and biochemical components associated with chilling tolerance in tomato: comparison of different developmental stages.Molecular horticulture · 2024Article
- Advancements in Reference Gene Selection for Fruit Trees: A Comprehensive Review.International journal of molecular sciences · 2024Review
- Drought and Oxidative Stress in Flax (Antioxidants (Basel, Switzerland) · 2023Article
- Effects of Light Quality on Colonization of Tomato Roots by AMF and Implications for Growth and Defense.Plants (Basel, Switzerland) · 2022Article
- Indolamine accumulation andFrontiers in plant science · 2022Article
- Selection and Evaluation of Candidate Reference Genes for Quantitative Real-Time PCR in Aboveground Tissues and Drought Conditions inFrontiers in genetics · 2022Article
- Coordinated transcriptional regulation of the carotenoid biosynthesis contributes to fruit lycopene content in high-lycopene tomato genotypes.Horticulture research · 2022Article
- Engineering high levels of saffron apocarotenoids in tomato.Horticulture research · 2022Article
- Increasing Fruit Weight by Editing aFrontiers in plant science · 2022Article
- Stability analysis of reference genes for RT-qPCR assays involving compatible and incompatible Ralstonia solanacearum-tomato 'Hawaii 7996' interactions.Scientific reports · 2021Article
- The Effects of Salinity on the Anatomy and Gene Expression Patterns in Leaflets of Tomato cv. Micro-Tom.Genes · 2021Article
- Article
- Structural and Functional Diversification of SEPALLATA Genes TM5 and RIN in Tomato Species (Section Lycopersicon).Doklady. Biochemistry and biophysics · 2020Article
- Fruit metabolic and transcriptional programs differentiate among Andean tomato (Solanum lycopersicum L.) accessions.Planta · 2019Article
- Selection and Validation of Novel RT-qPCR Reference Genes under Hormonal Stimuli and in Different Tissues of Santalum album.Scientific reports · 2018Article
- Evaluation of internal control genes for quantitative realtime PCR analyses for studying fruit development of dwarf tomato cultivar 'Micro-Tom'.Plant biotechnology (Tokyo, Japan) · 2018Article
- Reference Gene Selection for Quantitative Real-Time PCR of Mycelia fromBioMed research international · 2018Article
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5 authors.
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Abstract
Quantitative real-time RT-PCR (qRT-PCR) has become one of the most widely used methods for accurate quantification of gene expression. Since there are no universal reference genes for normalization, the optimal strategy to normalize raw qRT-PCR data is to perform an initial comparison of a set of independent reference genes to assess the most stable ones in each biological model. Normalization of a qRT-PCR experiment helps to ensure that the results are both statistically significant and biologically meaningful. Tomato is the model of choice to study fleshy fruit development. The miniature tomato (Solanum lycopersicum L.) cultivar Micro-Tom (MT) is considered a model system for tomato genetics and functional genomics. A new genotype, containing the Rg1 allele, improves tomato in vitro regeneration. In this work, we evaluated the expression stability of four tomato reference genes, namely CAC, SAND, Expressed, and ACTIN2. We showed that the genes CAC and Exp are the best reference genes of the four we tested during fruit development in the MT-Rg1 genotype. Furthermore, we validated the reference genes by showing that the expression profiles of the transcription factors FRUITFULL1 and APETALA2c during fruit development are comparable to previous reports using other tomato cultivars.
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