Evidence map›Paper›PMID 27679476›Full record

ArticleNucleic acids research2017

H3K9me3 demethylase Kdm4d facilitates the formation of pre-initiative complex and regulates DNA replication.

Rentian Wu, Zhiquan Wang, Honglian Zhang, Haiyun Gan, Zhiguo Zhang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nucleic acids research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.

0numbers the graph read from it
0cells of the map it votes in
35citing papers in PubMed
3.3field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

35 citing papers in PubMed, 57 citations in OpenAlex.

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  8. H3K9me3 Levels Affect the Proliferation of Bovine Spermatogonial Stem Cells.International journal of molecular sciences · 2024
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Rentian WuDepartment of Biochemistry and Molecular Biology, Mayo Clinic Cancer Center, Mayo Clinic, Rochester, MN 55902, USA.
Zhiquan WangDepartment of Biochemistry and Molecular Biology, Mayo Clinic Cancer Center, Mayo Clinic, Rochester, MN 55902, USA.
Honglian ZhangInstitute for Cancer Genetics, Department of Pediatric and Department of Genetics and Development, Columbia University, New York, NY 10032, USA.
Haiyun GanInstitute for Cancer Genetics, Department of Pediatric and Department of Genetics and Development, Columbia University, New York, NY 10032, USA.
Zhiguo ZhangInstitute for Cancer Genetics, Department of Pediatric and Department of Genetics and Development, Columbia University, New York, NY 10032, USA zz2401@cumc.columbia.edu.
Columbia University · USMayo Clinic in Arizona · US

Funding

Mechanisms of Epigenetic inheritanceR35GM118015 · NIGMS · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI Zhiguo Zhang · 2016 to 2026
$9.5M
Identify and characterize genes involved in X-chromosome inactivationR01GM099722 · NIGMS · MAYO CLINIC ROCHESTER · PI ZHANG, ZHIGUO · 2012 to 2015
$1.2M
NIGMS NIH HHS R01 GM099722NIGMS NIH HHS R35 GM118015
6 · The paper itself

Abstract

DNA replication is tightly regulated to occur once and only once per cell cycle. How chromatin, the physiological substrate of DNA replication machinery, regulates DNA replication remains largely unknown. Here we show that histone H3 lysine 9 demethylase Kdm4d regulates DNA replication in eukaryotic cells. Depletion of Kdm4d results in defects in DNA replication, which can be rescued by the expression of H3K9M, a histone H3 mutant transgene that reverses the effect of Kdm4d on H3K9 methylation. Kdm4d interacts with replication proteins, and its recruitment to DNA replication origins depends on the two pre-replicative complex components (origin recognition complex [ORC] and minichromosome maintenance [MCM] complex). Depletion of Kdm4d impairs the recruitment of Cdc45, proliferating cell nuclear antigen (PCNA), and polymerase δ, but not ORC and MCM proteins. These results demonstrate a novel mechanism by which Kdm4d regulates DNA replication by reducing the H3K9me3 level to facilitate formation of pre-initiative complex.

Indexed as

DNA ReplicationAnimalsCell CycleCell Cycle ProteinsCell Line, TumorCell NucleusChromatinDNA Polymerase IIIHCT116 CellsHeLa CellsHistonesHumansJumonji Domain-Containing Histone DemethylasesLamin Type BMethylationMiceCDC45 protein, humanCell Cycle ProteinsChromatinDNA Polymerase IIIHistonesJumonji Domain-Containing Histone DemethylasesKDM4D protein, humanLamin Type BMCM4 protein, humanMinichromosome Maintenance Complex Component 4MYC protein, humanProliferating Cell Nuclear AntigenProto-Oncogene Proteins c-mycRNA, Small Interfering

Identifiers

PMID27679476
PMCPMC5224507
OpenAlexW2525554683

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.