ArticleAsian journal of andrology
Morphometry and subpopulation structure of Holstein bull spermatozoa: variations in ejaculates and cryopreservation straws.
Article in Asian journal of andrology. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 54 citations in OpenAlex.
- Sperm dynamics in the female reproductive tract and their role in regulating fertility.The Journal of reproduction and development · 2026Review
- Dairy bull sperm subpopulation behaviour in frozen-thawed semen across breed, temperature, and thawing time.Veterinarni medicina · 2026Article
- Effect of Breed and Season in Buck Semen Cryopreservation: The Portuguese Animal Germplasm Bank.Veterinary sciences · 2024Article
- Kinematic and Morphometric Assessment of Fresh Semen, before, during and after Mating Period in Brahman Bulls.Animals : an open access journal from MDPI · 2023Article
- Kinetic vitrification: concepts and perspectives in animal sperm cryopreservation.Animal reproduction · 2023Review
- Testis-Specific Isoform of NaInternational journal of molecular sciences · 2022Review
- Influence of Fat-Soluble Vitamin Intramuscular Supplementation on Kinematic and Morphometric Sperm Parameters of Boar Ejaculates.Frontiers in veterinary science · 2022Article
- Sperm quality and morphometry characterization of cryopreserved canine sperm in ACP-106c or TRIS.Animal reproduction · 2022Article
- Sperm bauplan and function and underlying processes of sperm formation and selection.Physiological reviews · 2022Review
- Article
- Predictive Capacity of Boar Sperm Morphometry and Morphometric Sub-Populations on Reproductive Success after Artificial Insemination.Animals : an open access journal from MDPI · 2021Article
- Article
- Melatonin improves rate of monospermic fertilization and early embryo development in a bovine IVF system.PloS one · 2021Article
- Kinematic Sub-Populations in Bull Spermatozoa: A Comparison of Classical and Bayesian Approaches.Biology · 2020Article
- Fish Sperm Assessment Using Software and Cooling Devices.Journal of visualized experiments : JoVE · 2018Article
- Cluster analysis reveals seasonal variation of sperm subpopulations in extended boar semen.The Journal of reproduction and development · 2018Article
- Afterword toAsian journal of andrologyArticle
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sperm quality is evaluated for the calculation of sperm dosage in artificial reproductive programs. The most common parameter used is motility, but morphology has a higher potential as a predictor of genetic quality. Morphometry calculations from CASA-Morph technology improve morphological evaluation and allow mathematical approaches to the problem. Semen from 28 Holstein bulls was collected by artificial vagina, and several ejaculates were studied. After general evaluation, samples were diluted, packaged in 0.25 ml straws, and stored in liquid nitrogen. Two straws per sample were thawed, and slides were processed and stained with Diff-Quik. Samples were analyzed by a CASA-Morph system for eight morphometric parameters. In addition to the "classical" statistical approach, based on variance analysis (revealing differences between animals, ejaculates, and straws), principal component (PC) analysis showed that the variables were grouped into PC1, related to size, and PC2 to shape. Subpopulation structure analysis showed four groups, namely, big, small, short, and narrow from their dominant characteristics, representing 31.0%, 27.3%, 24.1%, and 17.7% of the total population, respectively. The distributions varied between animals and ejaculates, but between straws, there were no differences in only four animals. This modern approach of considering an ejaculate sperm population as divided into subpopulations reflecting quantifiable parameters generated by CASA-Morph systems technology opens a new view on sperm function. This is the first study applying this approach to evaluate different ejaculates and straws from the same individual. More work must be done to improve seminal dose calculations in assisted reproductive programs.
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