ArticleScientific reports2016
Persistence of fan-shaped keratocytes is a matrix-rigidity-dependent mechanism that requires α
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 39 citations in OpenAlex.
- Unlocking the therapeutic potential of cellular mechanobiology.Science advances · 2025Review
- Recent advances in shape memory scaffolds and regenerative outcomes.Biomedical engineering letters · 2024Review
- A minimal physical model for curvotaxis driven by curved protein complexes at the cell's leading edge.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Nascent adhesions shorten the period of lamellipodium protrusion through the Brownian ratchet mechanism.Molecular biology of the cell · 2023Article
- Matrix stiffness exacerbates the proinflammatory responses of vascular smooth muscle cell through the DDR1-DNMT1 mechanotransduction axis.Bioactive materials · 2022Article
- Unified multiscale theory of cellular mechanical adaptations to substrate stiffness.Biophysical journal · 2022Article
- Focal adhesion-mediated cell anchoring and migration: from in vitro to in vivo.Development (Cambridge, England) · 2022Article
- Appreciating the role of cell shape changes in the mechanobiology of epithelial tissues.Biophysics reviews · 2022Review
- Multiscale Mechanobiology in Brain Physiology and Diseases.Frontiers in cell and developmental biology · 2022Review
- Tumorigenic mesenchymal clusters are less sensitive to moderate osmotic stresses due to low amounts of junctional E-cadherin.Scientific reports · 2021Article
- Collective migration during a gap closure in a two-dimensional haptotactic model.Scientific reports · 2021Article
- A minimal mechanosensing model predicts keratocyte evolution on flexible substrates.Journal of the Royal Society, Interface · 2020Article
- Producing Collagen Micro-stripes with Aligned Fibers for Cell Migration Assays.Cellular and molecular bioengineering · 2020Article
- Actomyosin contractility scales with myoblast elongation and enhances differentiation through YAP nuclear export.Scientific reports · 2019Article
- Unchain My Heart: Integrins at the Basis of iPSC Cardiomyocyte Differentiation.Stem cells international · 2019Review
- Innovative Tools for Mechanobiology: Unraveling Outside-In and Inside-Out Mechanotransduction.Frontiers in bioengineering and biotechnology · 2019Review
- Keratocytes Generate High Integrin Tension at the Trailing Edge to Mediate Rear De-adhesion during Rapid Cell Migration.iScience · 2018Article
- Single and collective cell migration: the mechanics of adhesions.Molecular biology of the cell · 2017Article
- Eukaryotic Cell Dynamics from Crawlers to Swimmers.Wiley interdisciplinary reviews. Computational molecular scienceArticle
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Despite the importance of matrix rigidity on cell functions, many aspects of the mechanosensing process in highly migratory cells remain elusive. Here, we studied the migration of highly motile keratocytes on culture substrates with similar biochemical properties and rigidities spanning the range between soft tissues (~kPa) and stiff culture substrates (~GPa). We show that morphology, polarization and persistence of motile keratocytes are regulated by the matrix stiffness over seven orders of magnitude, without changing the cell spreading area. Increasing the matrix rigidity leads to more F-actin in the lamellipodia and to the formation of mature contractile actomyosin fibers that control the cell rear retraction. Keratocytes remain rounded and form nascent adhesions on compliant substrates, whereas large and uniformly distributed focal adhesions are formed on fan-shaped keratocytes migrating on rigid surfaces. By combining poly-L-lysine, fibronectin and vitronectin coatings with selective blocking of α
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.