ArticleThe Plant cell2016
Arabidopsis TH2 Encodes the Orphan Enzyme Thiamin Monophosphate Phosphatase.
Article in The Plant cell, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 42 citations in OpenAlex.
- ZmTH1 Is Vital for Healthy Plant Growth and Promotes Cold/Drought Tolerance by Regulating Thiamin Diphosphate-Dependent Metabolisms in Maize.Plant biotechnology journal · 2026Article
- In silico, in vitro, and in vivo characterization of thiamin-binding proteins from plant seeds.The Biochemical journal · 2025Article
- OsTH1 is a key player in thiamin biosynthesis in rice.Scientific reports · 2024Article
- HDF1, a novel flowering time regulator identified in a mutant suppressing sensitivity to red light reduced 1 early flowering.Scientific reports · 2023Article
- Roles of type II HFrontiers in plant science · 2023Article
- B vitamin supply in plants and humans: the importance of vitamer homeostasis.The Plant journal : for cell and molecular biology · 2022Review
- A novel panel of yeast assays for the assessment of thiamin and its biosynthetic intermediates in plant tissues.The New phytologist · 2022Article
- Natural Variation in Vitamin BFrontiers in plant science · 2022Article
- A Core Metabolome Response of Maize Leaves Subjected to Long-Duration Abiotic Stresses.Metabolites · 2021Article
- Metabolic engineering provides insight into the regulation of thiamin biosynthesis in plants.Plant physiology · 2021Article
- Metabolic engineering of rice endosperm towards higher vitamin B1 accumulation.Plant biotechnology journal · 2021Article
- Antioxidants in Potatoes: A Functional View on One of the Major Food Crops Worldwide.Molecules (Basel, Switzerland) · 2021Review
- The rice PALE1 homolog is involved in the biosynthesis of vitamin B1.Plant biotechnology journal · 2021Article
- TheFrontiers in plant science · 2021Article
- The importance of thiamine (vitamin BThe Journal of biological chemistry · 2020Review
- On the nature of thiamine triphosphate in Arabidopsis.Plant direct · 2020Article
- The coenzyme thiamine diphosphate displays a daily rhythm in the Arabidopsis nucleus.Communications biology · 2020Article
- Maize transposable elements contribute to long non-coding RNAs that are regulatory hubs for abiotic stress response.BMC genomics · 2019Article
- Appropriate Thiamin Pyrophosphate Levels Are Required for Acclimation to Changes in Photoperiod.Plant physiology · 2019Article
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Authors and funding
14 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To synthesize the cofactor thiamin diphosphate (ThDP), plants must first hydrolyze thiamin monophosphate (ThMP) to thiamin, but dedicated enzymes for this hydrolysis step were unknown and widely doubted to exist. The classical thiamin-requiring th2-1 mutation in Arabidopsis thaliana was shown to reduce ThDP levels by half and to increase ThMP levels 5-fold, implying that the THIAMIN REQUIRING2 (TH2) gene product could be a dedicated ThMP phosphatase. Genomic and transcriptomic data indicated that TH2 corresponds to At5g32470, encoding a HAD (haloacid dehalogenase) family phosphatase fused to a TenA (thiamin salvage) family protein. Like the th2-1 mutant, an insertional mutant of At5g32470 accumulated ThMP, and the thiamin requirement of the th2-1 mutant was complemented by wild-type At5g32470 Complementation tests in Escherichia coli and enzyme assays with recombinant proteins confirmed that At5g32470 and its maize (Zea mays) orthologs GRMZM2G148896 and GRMZM2G078283 are ThMP-selective phosphatases whose activity resides in the HAD domain and that the At5g32470 TenA domain has the expected thiamin salvage activity. In vitro and in vivo experiments showed that alternative translation start sites direct the At5g32470 protein to the cytosol and potentially also to mitochondria. Our findings establish that plants have a dedicated ThMP phosphatase and indicate that modest (50%) ThDP depletion can produce severe deficiency symptoms.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.