ArticleScientific reports2016
Improving protein content and quality by over-expressing artificially synthetic fusion proteins with high lysine and threonine constituent in rice plants.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 60 citations in OpenAlex.
- Rational gene stacking for seed-specific carbon partitioning to simultaneously enhance lipid and protein contents in Arabidopsis.Journal of experimental botany · 2026Article
- Genetically Modified Plants in Agriculture.Biology · 2026Review
- Fusion protein based strategies for developing insect-resistant crops.Functional & integrative genomics · 2026Review
- CRISPR/Cas9-based editing of NF-YC4 promoters yields high-protein rice and soybean.The New phytologist · 2025Article
- Grain Chalkiness Is Decreased by Balancing the Synthesis of Protein and Starch in Hybrid Indica Rice Grains under Nitrogen Fertilization.Foods (Basel, Switzerland) · 2024Article
- Genetically Modified Rice Is Associated with Hunger, Health, and Climate Resilience.Foods (Basel, Switzerland) · 2023Review
- Variations in Total Protein and Amino Acids in the Sequenced Sorghum Mutant Library.Plants (Basel, Switzerland) · 2023Article
- Engineering a feedback inhibition-insensitive plant dihydrodipicolinate synthase to increase lysine content in Camelina sativa seeds.Transgenic research · 2022Article
- Lactic Acid Bacteria-Fermentable Cereal- and Pseudocereal-Based Beverages.Microorganisms · 2021Review
- Article
- SUPER STARCHY1/ONAC025 participates in rice grain filling.Plant direct · 2020Article
- OsASN1 Overexpression in Rice Increases Grain Protein Content and Yield under Nitrogen-Limiting Conditions.Plant & cell physiology · 2020Article
- The application of gene splitting technique for controlling transgene flow in rice.Transgenic research · 2020Article
- Genetic Manipulation for Improved Nutritional Quality in Rice.Frontiers in genetics · 2020Review
- Applications of the CRISPR/Cas9 System for Rice Grain Quality Improvement: Perspectives and Opportunities.International journal of molecular sciences · 2019Review
- Genome-wide identification and characterization of seed storage proteins (SSPs) of foxtail millet (3 Biotech · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rice grains are rich in starch but low in protein with very low level of both lysine and threonine. Thus, it is important to further improve protein quality and quantity, especially to increase lysine and threonine content in rice grains. We artificially synthesized two new genes by fusing endogenous rice genes with lysine (K)/threonine (T) motif (TKTKK) coding sequences. They were designated as TKTKK1 and TKTKK2 and their encoded proteins consist of 73.1% and 83.5% of lysine/threonine, respectively. These two genes were under the control of 35S promoter and were independently introduced into the rice genome to generate transgenic plants. Our data showed that overexpression of TKTKK1 generated stable proteins with expected molecular weight and the transgenic rice seeds significantly increased lysine, threonine, total amino acids and crude protein content by 33.87%, 21.21%, 19.43% and 20.45%, respectively when compared with wild type control; significant improvement was also observed in transgenic rice seeds overexpressing TKTKK2. However, limited improvement in protein quality and quantity was observed in transgenic seeds carrying tandom array of these two new genes. Our data provide the basis and alternative strategy on further improving protein quality and quantity in other crops or vegetable plants by synthetic biology.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.