ArticleInternational urology and nephrology2017
Effect of pH on the isolation of urinary exosome.
Article in International urology and nephrology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Urinary extracellular vesicles: A position paper by the Urine Task Force of the International Society for Extracellular Vesicles.Journal of extracellular vesicles · 2021Guideline
- A biomimetic cryoprotectant preserves extracellular vesicles bioactivity for intravenous administration and potentiates wound healing.Journal of nanobiotechnology · 2026Article
- Exosomes as a biomarker in postoperative complications: a literature review.Frontiers in cell and developmental biology · 2026Review
- Cutting-Edge Progress in the Acquisition, Modification and Therapeutic Applications of Exosomes for Drug Delivery.International journal of nanomedicine · 2025Review
- Research progress of extracellular vesicles derived from mesenchymal stem cells in the treatment of neurodegenerative diseases.Frontiers in immunology · 2025Review
- Optimization and Characterization of PEG Extraction Process for Tartary Buckwheat-Derived Nanoparticles.Foods (Basel, Switzerland) · 2024Article
- Plant-derived vesicle-like nanoparticles: A new tool for inflammatory bowel disease and colitis-associated cancer treatment.Molecular therapy : the journal of the American Society of Gene Therapy · 2024Review
- Polyethylene glycol-based isolation of urinary extracellular vesicles, an easily adoptable protocol.MethodsX · 2023Article
- Extracellular Vesicles in Neurodegenerative Diseases: An Update.International journal of molecular sciences · 2023Review
- Review of the Role of Mesenchymal Stem Cells and Exosomes Derived from Mesenchymal Stem Cells in the Treatment of Orthopedic Disease.Medical science monitor : international medical journal of experimental and clinical research · 2022Review
- Low pH-Based Method to Increase the Yield of Plant-Derived Nanoparticles from Fresh Ginger Rhizomes.ACS omega · 2021Article
- Current Strategies for Cancer Cell-Derived Extracellular Vesicles for Cancer Therapy.Frontiers in oncology · 2021Review
- Type I collagen hydrogels as a delivery matrix for royal jelly derived extracellular vesicles.Drug delivery · 2020Article
- Zeta Potential of Extracellular Vesicles: Toward Understanding the Attributes that Determine Colloidal Stability.ACS omega · 2020Article
- Extracellular vesicles enhance oxidative stress through P38/NF-kB pathway in ketamine-induced ulcerative cystitis.Journal of cellular and molecular medicine · 2020Article
- Effect of pH, temperature and freezing-thawing on quantity changes and cellular uptake of exosomes.Protein & cell · 2019Article
- Exosomal ephrinA2 derived from serum as a potential biomarker for prostate cancer.Journal of Cancer · 2018Article
- Alteration of exosomes secreted from renal tubular epithelial cells exposed to high-concentration oxalate.Oncotarget · 2017Article
- Extracellular Vesicles in Renal Pathophysiology.Frontiers in molecular biosciences · 2017Review
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeUrinary exosome is an ideal noninvasive, low-cost source of kidney diseases biomarkers. However, many factors effect the isolation of urine-derived exosome. The effect of pH on the yield of exosome isolation under different pH was explored.
methodsThe pH was adjusted for 4, 5, 6, 7 and 8 with hydrochloric acid and sodium hydroxide solution. All samples were incubated for 30 min before differential ultracentrifugation. Exosome-associated protein markers TSG101, ALIX and total concentration of RNA were evaluated by Western-blotting and micro-amount ultraviolet spectrophotometer, respectively.
resultsA major loss of urinary exosome was received at pH 8 compared with alkali medium or control group. There was no difference whether or not added EDTA-Na
conclusionsAcidic environment was likely to conducive to the isolation of exosome and maintain its stability and integrity that suggest pH medium needs to be carefully considered and also provide a methodology for future separation exosome.
Indexed as
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.