ArticleNature communications2016
Forward design of a complex enzyme cascade reaction.
Article in Nature communications, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
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Who cites it
32 citing papers in PubMed, 103 citations in OpenAlex.
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- Quantitative Online Monitoring of an Immobilized Enzymatic Network by Ion Mobility-Mass Spectrometry.Journal of the American Chemical Society · 2024Article
- Exploring Emergent Properties in Enzymatic Reaction Networks: Design and Control of Dynamic Functional Systems.Chemical reviews · 2024Review
- Iterative design of training data to control intricate enzymatic reaction networks.Nature communications · 2024Article
- EnzymeMap: curation, validation and data-driven prediction of enzymatic reactions.Chemical science · 2023Article
- Artificial multi-enzyme cascades and whole-cell transformation for bioconversion of C1 compounds: Advances, challenge and perspectives.Synthetic and systems biotechnology · 2023Review
- A closed-loop catalytic nanoreactor system on a transistor.Science advances · 2023Article
- Time-resolved multiparameter analytics on a cell-free production platform for acyl-CoA precursors.Analytical science advances · 2022Article
- Controllable Iterative β-Glucosylation from UDP-Glucose byJournal of agricultural and food chemistry · 2021Article
- EHreact: Extended Hasse Diagrams for the Extraction and Scoring of Enzymatic Reaction Templates.Journal of chemical information and modeling · 2021Article
- Elevated, non-proliferative temperatures change the profile of fermentation products in Corynebacterium glutamicum.Applied microbiology and biotechnology · 2021Article
- Cell-free synthesis system-assisted pathway bottleneck diagnosis and engineering inSynthetic and systems biotechnology · 2020Article
- In vitro prototyping and rapid optimization of biosynthetic enzymes for cell design.Nature chemical biology · 2020Article
- 2-Ketoglutarate-Generated In Vitro Enzymatic Biosystem Facilitates Fe(II)/2-Ketoglutarate-Dependent Dioxygenase-Mediated C-H Bond Oxidation for (2International journal of molecular sciences · 2020Article
- Intercellular communication between artificial cells by allosteric amplification of a molecular signal.Nature communications · 2020Article
- Supramolecular chemistry under mechanochemical conditions: a small molecule template generated and integrated into a molecular-to-supramolecular and back-to-molecular cascade reaction.Chemical science · 2020Article
- A combined experimental and modelling approach for the Weimberg pathway optimisation.Nature communications · 2020Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Enzymatic reaction networks are unique in that one can operate a large number of reactions under the same set of conditions concomitantly in one pot, but the nonlinear kinetics of the enzymes and the resulting system complexity have so far defeated rational design processes for the construction of such complex cascade reactions. Here we demonstrate the forward design of an in vitro 10-membered system using enzymes from highly regulated biological processes such as glycolysis. For this, we adapt the characterization of the biochemical system to the needs of classical engineering systems theory: we combine online mass spectrometry and continuous system operation to apply standard system theory input functions and to use the detailed dynamic system responses to parameterize a model of sufficient quality for forward design. This allows the facile optimization of a 10-enzyme cascade reaction for fine chemical production purposes.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.