ArticleZebrafish2017
zWEDGI: Wounding and Entrapment Device for Imaging Live Zebrafish Larvae.
Article in Zebrafish, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
37 citing papers in PubMed, 55 citations in OpenAlex.
- GAPDH nuclear signaling regulates neutrophil migration and inflammatory skin responses.Research square · 2026Article
- Fibroblast response to burn injury in larval zebrafish mirrors developmental maturation and is inhibited by infiltrating neutrophils.Development (Cambridge, England) · 2026Article
- Real-time characterisation of microbe-induced inflammation using a novel zebrafish larval corneal injury and infection model.Communications biology · 2026Article
- Evidence that xylazine disrupts skin homeostasis by acting on epithelial cells through the kappa opioid receptor.Disease models & mechanisms · 2026Article
- Assessing Intracellular Metabolism of Immune Cells In Situ in Live Zebrafish Larvae by Autofluorescence Lifetime Imaging Microscopy of NAD(P)H and FAD.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Azole drugs have differential efficacy in varied models of immunosuppression in larval zebrafish hosts.Future microbiology · 2025Article
- Macrophage vacuolar ATPase (v-ATPase) function controlsbioRxiv : the preprint server for biology · 2025Article
- Co-zorbs: Motile, multispecies biofilms aid transport of diverse bacterial species.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Real-time imaging reveals a role for macrophage protrusive motility in melanoma invasion.The Journal of cell biology · 2025Article
- Agarose stamped method: a simple and customizable immobilization technique for zebrafish larvae.Frontiers in behavioral neuroscience · 2025Article
- Single cell autofluorescence imaging reveals immediate metabolic shifts of neutrophils with activation across biological systems.Frontiers in immunology · 2025Article
- Glucocorticoids Suppress NF-κB-Mediated Neutrophil Control of Aspergillus fumigatus Hyphal Growth.Journal of immunology (Baltimore, Md. : 1950) · 2024Article
- Low-cost Polyethylene Terephthalate Lamination Microfluidics Designs for Multiplexed Zebrafish Imaging.Journal of visualized experiments : JoVE · 2024Article
- Staphylococcus aureus lipid factors modulate melanoma cell clustering and invasion.Disease models & mechanisms · 2024Article
- Article
- Co-zorbs: Motile, multispecies biofilms aid transport of diverse bacterial species.bioRxiv : the preprint server for biology · 2024Article
- Macrophages inhibit extracellular hyphal growth ofInfection and immunity · 2024Article
- Aspergillus fumigatus transcription factor ZfpA regulates hyphal development and alters susceptibility to antifungals and neutrophil killing during infection.PLoS pathogens · 2023Article
- Article
- Cyclooxygenase production of PGE2 promotes phagocyte control of A. fumigatus hyphal growth in larval zebrafish.PLoS pathogens · 2022Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
Zebrafish, an established model organism in developmental biology, is also a valuable tool for imaging wound healing in space and time with cellular resolution. However, long-term imaging of wound healing poses technical challenges as wound healing occurs over multiple temporal scales. The traditional strategy of larval encapsulation in agarose successfully limits sample movement but impedes larval development and tissue regrowth and is therefore not amenable to long-term imaging of wound healing. To overcome this challenge, we engineered a functionally compartmentalized device, the zebrafish Wounding and Entrapment Device for Growth and Imaging (zWEDGI), to orient larvae for high-resolution microscopy, including confocal and second harmonic generation (SHG), while allowing unrestrained tail development and regrowth. In this device, larval viability was maintained and tail regrowth was improved over embedding in agarose. The quality of tail fiber SHG images collected from larvae in the device was similar to fixed samples but provided the benefit of time lapse data collection. Furthermore, we show that this device was amenable to long-term (>24 h) confocal microscopy of the caudal fin. Finally, the zWEDGI was designed and fabricated using readily available techniques so that it can be easily modified for diverse experimental imaging protocols.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.