ArticlePeerJ2016
Metaproteomics of saliva identifies human protein markers specific for individuals with periodontitis and dental caries compared to orally healthy controls.
Article in PeerJ, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers, 1 of them a synthesis that pooled it.
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The trial behind it
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Who cites it
36 citing papers in PubMed, 1 synthesis or guideline pooled it, 75 citations in OpenAlex.
- Salivary Proteomics for Detecting Novel Biomarkers of Periodontitis: A Systematic Review.Journal of periodontal research · 2025Pooled it
- Trial
- Oral Cavity Antibacterial Discovery Pipeline Driven by Advanced Analytical Techniques.Molecules (Basel, Switzerland) · 2026Review
- Obstructive Sleep Apnea Associated with Periodontitis: Evidence from Mendelian Randomization and Salivary Proteomics.Journal of proteome research · 2026Article
- A Viral Mutation Profiling and Discovery Strategy for Sensitive Multiplex Detection of Viruses and Variants in Saliva by Proteomics.bioRxiv : the preprint server for biology · 2026Article
- Salivary immune alteration persists after caries treatment.Scientific reports · 2026Article
- Multi-Omics Insights into Gingivitis from a Clinical Trial: Understanding the Role of Bacterial and Host Factors.Microorganisms · 2025Article
- OSaMPle workflow for salivary metaproteomics analysis reveals dysbiosis in inflammatory bowel disease patients.NPJ biofilms and microbiomes · 2025Article
- Salivary proteomics and metaproteomics identifies distinct molecular and taxonomic signatures of type-2 diabetes.Microbiome · 2025Article
- Probing the salivary proteome for prognostic biomarkers in response to non-surgical periodontal therapy.Journal of clinical periodontology · 2025Article
- Symbiosis Between the Oral Microbiome and the Human Host: Microbial Homeostasis and Stability of the Host.Advances in experimental medicine and biology · 2025Review
- A Study of the Association between Primary Oral Pathologies (Dental Caries and Periodontal Diseases) Using Synchrotron Molecular FTIR Spectroscopy in View of the Patient's Personalized Clinical Picture (Demographics and Anamnesis).International journal of molecular sciences · 2024Article
- Omics for deciphering oral microecology.International journal of oral science · 2024Review
- Unlocking the Diagnostic Potential of Saliva: A Comprehensive Review of Infrared Spectroscopy and Its Applications in Salivary Analysis.Journal of personalized medicine · 2023Review
- Oral mucosal breaks trigger anti-citrullinated bacterial and human protein antibody responses in rheumatoid arthritis.Science translational medicine · 2023Article
- Current State and Challenges of the Global Outcomes of Dental Caries Research in the Meta-Omics Era.Frontiers in cellular and infection microbiology · 2022Review
- The oral microbiome: Role of key organisms and complex networks in oral health and disease.Periodontology 2000 · 2021Review
- Metagenomic analysis of the salivary microbiota in patients with caries, periodontitis and comorbid diseases.Journal of dental sciences · 2021Article
- Salivary proteome of aphthous stomatitis reveals the participation of vitamin metabolism, nutrients, and bacteria.Scientific reports · 2021Article
- Metagenomic and Metatranscriptomic Insight Into Oral Biofilms in Periodontitis and Related Systemic Diseases.Frontiers in microbiology · 2021Review
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe composition of the salivary microbiota has been reported to differentiate between patients with periodontitis, dental caries and orally healthy individuals. To identify characteristics of diseased and healthy saliva we thus wanted to compare saliva metaproteomes from patients with periodontitis and dental caries to healthy individuals.
methodsStimulated saliva samples were collected from 10 patients with periodontitis, 10 patients with dental caries and 10 orally healthy individuals. The proteins in the saliva samples were subjected to denaturing buffer and digested enzymatically with LysC and trypsin. The resulting peptide mixtures were cleaned up by solid-phase extraction and separated online with 2 h gradients by nano-scale C18 reversed-phase chromatography connected to a mass spectrometer through an electrospray source. The eluting peptides were analyzed on a tandem mass spectrometer operated in data-dependent acquisition mode.
resultsWe identified a total of 35,664 unique peptides from 4,161 different proteins, of which 1,946 and 2,090 were of bacterial and human origin, respectively. The human protein profiles displayed significant overexpression of the complement system and inflammatory markers in periodontitis and dental caries compared to healthy controls. Bacterial proteome profiles and functional annotation were very similar in health and disease.
conclusionsOverexpression of proteins related to the complement system and inflammation seems to correlate with oral disease status. Similar bacterial proteomes in healthy and diseased individuals suggests that the salivary microbiota predominantly thrives in a planktonic state expressing no disease-associated characteristics of metabolic activity.
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