Evidence map›Paper›PMID 27671873›Full record

ArticleDevelopmental biology2016

Three-dimensional microCT imaging of mouse development from early post-implantation to early postnatal stages.

Chih-Wei Hsu, Leeyean Wong, Tara L Rasmussen, Sowmya Kalaga, Melissa L McElwee, Lance C Keith, Ritu Bohat, John R Seavitt, Arthur L Beaudet, Mary E Dickinson

Abstract readVideo-Audio Media
In one paragraph

Article in Developmental biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.

0numbers the graph read from it
0cells of the map it votes in
37citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

37 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
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  5. Article
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  9. Buffered Lugol's Iodine Preserves DNA Fragment Lengths.Integrative organismal biology (Oxford, England) · 2024
    Article
  10. Three-dimensional microCT imaging of mouse heart development from early post-implantation to late fetal stages.Mammalian genome : official journal of the International Mammalian Genome Society · 2023
    Review
  11. Article
  12. Article
  13. Early construction of the thalamocortical axon pathway requires c-Jun N-terminal kinase signaling within the ventral forebrain.Developmental dynamics : an official publication of the American Association of Anatomists · 2022
    Article
  14. Article
  15. Mouse embryo phenotyping using X-ray microCT.Frontiers in cell and developmental biology · 2022
    Review
  16. Maternal background alters the penetrance of growth phenotypes and sex-specific placental adaptation of offspring sired by alcohol-exposed males.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021
    Article
  17. Article
  18. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Chih-Wei HsuDepartment of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, TX 77030, USA; Optical Imaging and Vital Microscopy Core, Baylor College of Medicine, Houston, TX 77030, USA.
Leeyean WongDepartment of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, TX 77030, USA.
Tara L RasmussenDepartment of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, TX 77030, USA.
Sowmya KalagaDepartment of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, TX 77030, USA; Optical Imaging and Vital Microscopy Core, Baylor College of Medicine, Houston, TX 77030, USA.
Melissa L McElweeDepartment of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, TX 77030, USA.
Lance C KeithDepartment of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, TX 77030, USA.
Ritu BohatDepartment of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA.
John R SeavittDepartment of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA.
Arthur L BeaudetDepartment of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA.
Mary E DickinsonDepartment of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, TX 77030, USA; Optical Imaging and Vital Microscopy Core, Baylor College of Medicine, Houston, TX 77030, USA; Cardiovascular Research Institute, Baylor College of Medicine, Houston, TX 77030, USA. Electronic address: mdickins@bcm.edu.

Funding

UM1HG006348: Cas9 Genome Integrity Supplemental ProposalUM1HG006348 · NHGRI · BAYLOR COLLEGE OF MEDICINE · PI Jason D. Heaney, Chih-Wei Logan Hsu · 2016 to 2026
$47.5M
Consortium for Broad Based Disease Phenotyping of Knockout MiceU54HG006348 · NHGRI · BAYLOR COLLEGE OF MEDICINE · PI BEAUDET, ARTHUR L., PAYLOR, RICHARD E · 2011 to 2015
$17.0M
Biomechanics of early mammalian cardiogenesisR01HL120140 · NHLBI · BAYLOR COLLEGE OF MEDICINE · PI LARINA, IRINA · 2013 to 2017
$2.0M
NHGRI NIH HHS U54 HG006348NHGRI NIH HHS UM1 HG006348NHLBI NIH HHS R01 HL120140
6 · The paper itself

Abstract

In this work, we report the use of iodine-contrast microCT to perform high-throughput 3D morphological analysis of mouse embryos and neonates between embryonic day 8.5 to postnatal day 3, with high spatial resolution up to 3µm/voxel. We show that mouse embryos at early stages can be imaged either within extra embryonic tissues such as the yolk sac or the decidua without physically disturbing the embryos. This method enables a full, undisturbed analysis of embryo turning, allantois development, vitelline vessels remodeling, yolk sac and early placenta development, which provides increased insights into early embryonic lethality in mutant lines. Moreover, these methods are inexpensive, simple to learn and do not require substantial processing time, making them ideal for high throughput analysis of mouse mutants with embryonic and early postnatal lethality.

Indexed as

Embryonic DevelopmentAnimalsAnimals, NewbornContrast MediaDeciduaFemaleGenes, LethalGenetic Association StudiesGestational AgeHydrogelsImaging, Three-DimensionalIodineMicePhenotypeStaining and LabelingX-Ray MicrotomographyContrast MediaHydrogelsIodineEmbryonic lethal screeningIMPCMicroCT

Identifiers

PMID27671873
PMCPMC5405732

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.