ArticleProceedings of the National Academy of Sciences of the United States of America2016
Mitochondrial DNA from the eradicated European Plasmodium vivax and P. falciparum from 70-year-old slides from the Ebro Delta in Spain.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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29 citing papers in PubMed, 60 citations in OpenAlex.
- Ancient DNA analyses of remains of the Medici family (16iScience · 2026Article
- Intra-individual variability in ancient plasmodium DNA recovery highlights need for enhanced sampling.Scientific reports · 2025Article
- Genomic exploration of the journey of Plasmodium vivax in Latin America.PLoS pathogens · 2025Article
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- Pathogen prospecting of museums: Reconstructing malaria epidemiology.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Use of rapid diagnostic tests for the detection of ancient malaria infections in dental pulp from the sixth century in Versailles, France.Malaria journal · 2023Article
- Geographical origin of Plasmodium vivax in the Hainan Island, China: insights from mitochondrial genome.Malaria journal · 2023Article
- NovelLancet regional health. Americas · 2023Article
- From modern-day parasitology to paleoparasitology: the elusive past record and evolution ofFrontiers in microbiology · 2023Review
- Evolutionary history of Plasmodium vivax and Plasmodium simium in the Americas.Malaria journal · 2022Review
- The genome of the zoonotic malaria parasite Plasmodium simium reveals adaptations to host switching.BMC biology · 2021Article
- The epidemiology of Plasmodium vivax among adults in the Democratic Republic of the Congo.Nature communications · 2021Article
- The elusive parasite: comparing macroscopic, immunological, and genomic approaches to identifying malaria in human skeletal remains from Sayala, Egypt (third to sixth centuries AD).Archaeological and anthropological sciences · 2021Article
- Intestinal helminths as a biomolecular complex in archaeological research.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2020Article
- Ape Origins of Human Malaria.Annual review of microbiology · 2020Review
- Laboratory colonization and maintenance of Anopheles atroparvus from the Ebro Delta, Spain.Parasites & vectors · 2020Article
- Metagenomic analysis of a blood stain from the French revolutionary Jean-Paul Marat (1743-1793).Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2020Article
- Plasmodium vivax Malaria Viewed through the Lens of an Eradicated European Strain.Molecular biology and evolution · 2020Article
- Human Plasmodium vivax diversity, population structure and evolutionary origin.PLoS neglected tropical diseases · 2020Article
- Genetic affinities of an eradicated EuropeanMicrobial genomics · 2019Article
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Authors and funding
11 authors at 7 institutions in 7 countries.
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Abstract
Phylogenetic analysis of Plasmodium parasites has indicated that their modern-day distribution is a result of a series of human-mediated dispersals involving transport between Africa, Europe, America, and Asia. A major outstanding question is the phylogenetic affinity of the malaria causing parasites Plasmodium vivax and falciparum in historic southern Europe-where it was endemic until the mid-20th century, after which it was eradicated across the region. Resolving the identity of these parasites will be critical for answering several hypotheses on the malaria dispersal. Recently, a set of slides with blood stains of malaria-affected people from the Ebro Delta (Spain), dated between 1942 and 1944, have been found in a local medical collection. We extracted DNA from three slides, two of them stained with Giemsa (on which Plasmodium parasites could still be seen under the microscope) and another one consisting of dried blood spots. We generated the data using Illumina sequencing after using several strategies aimed at increasing the Plasmodium DNA yield: depletion of the human genomic (g)DNA content through hybridization with human gDNA baits, and capture-enrichment using gDNA derived from P. falciparum Plasmodium mitochondrial genome sequences were subsequently reconstructed from the resulting data. Phylogenetic analysis of the eradicated European P. vivax mtDNA genome indicates that the European isolate is closely related to the most common present-day American haplotype and likely entered the American continent post-Columbian contact. Furthermore, the European P. falciparum mtDNA indicates a link with current Indian strains that is in agreement with historical accounts.
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