ArticleScientific reports2016
Co-stimulation of HaCaT keratinization with mechanical stress and air-exposure using a novel 3D culture device.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed.
- Identification and functional assessment of CDSN gene variants associated with horn phenotypes in sheep.BMC genomics · 2026Article
- Electrospun 3D Nanofiber Scaffolds with Adhesion-Enhanced Interfaces for Cell Culture.ACS omega · 2026Article
- The Role of Collagen Rheology in Human Keratinocyte Differentiation: Implications for Skin Substitute Development.Polymers · 2025Article
- Dysregulation of STS in keratinocytes promotes calcium signaling and differentiation.Scientific reports · 2025Article
- Breaching the Barrier: Investigating Initial Herpes Simplex Viral Infection and Spread in Human Skin and Mucosa.Viruses · 2024Review
- From static to dynamic: The influence of mechanotransduction on skin equivalents analyzed by bioimaging and RNAseq.Materials today. Bio · 2024Article
- Skin-on-a-Chip Technology: Microengineering Physiologically Relevant In Vitro Skin Models.Pharmaceutics · 2022Review
- Steroid sulfatase deficiency causes cellular senescence and abnormal differentiation by inducing Yippee-like 3 expression in human keratinocytes.Scientific reports · 2021Article
- Article
- Profilin-1; a novel regulator of DNA damage response and repair machinery in keratinocytes.Molecular biology reports · 2021Article
- PEG grafted chitosan scaffold for dual growth factor delivery for enhanced wound healing.Scientific reports · 2019Article
- An organotypic oral mucosal infection model to study host-pathogen interactions.Journal of tissue engineeringArticle
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Artificial skin or skin equivalents have been used for clinical purpose to skin graft and as substitutes for animal experiments. The culture of cell lines such as HaCaT has the potential to produce large amounts of artificial skin at a low cost. However, there is a limit to keratinization due to the restriction of differentiation in HaCaT. In this study, a culture device that mimics the in vivo keratinization mechanism, co-stimulated by air-exposure and mechanical stimulation, was developed to construct skin equivalents. The device can reconstruct the epidermal morphology, including the cornified layer, similar to its formation in vivo. Under the condition, epidermis was differentiated in the spinous and granular layers. Formation of the stratum corneum is consistent with the mRNA and protein expressions of differentiation markers. The device is the first of its kind to combine air-exposure with mechanical stress to co-stimulate keratinization, which can facilitate the economically viable production of HaCaT-based artificial skin substitutes.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.