Evidence map›Paper›PMID 27670109›Full record

Trial reportNature microbiology2016

Enrichment of the lung microbiome with gut bacteria in sepsis and the acute respiratory distress syndrome.

Robert P Dickson, Benjamin H Singer, Michael W Newstead, Nicole R Falkowski, John R Erb-Downward, Theodore J Standiford, Gary B Huffnagle

2 registry-linked trialsAbstract readMulticenter StudyRandomized Controlled Trial
PubMed Full text at PMC Publisher
In one paragraph

Trial report in Nature microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to 2 registered trials, which are not on this map. Cited by 429 papers, 6 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
429citing papers in PubMed, 6 pooled it
17.9field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT04032938 unknown statusnot on this mapstarted 2018, after this paper: background citation

Clinical and Multi-omics Study on Gut Microbiota in Critical Ill Patients After Cardiovascular Surgery Combined Cardiopulmonary Bypass With or Without Sepsis（CPB-MUL-GM Study): a Prospective Study Protocol

TypeobservationalSponsorPeking Union Medical College HospitalRan2018 to 2019Enrolled150ConditionsSepsis, Cardiopulmonary BypassArmsextracorporeal circulation during cardiac surgery
NCT04598334 unknown statusnot on this mapstarted 2022, after this paper: background citation

Investigating the Inflammatory Cytokines and Cytokine Storm Among Bangladeshi Patients With COVID-19: a Prospective, Observational Study

TypeobservationalSponsorInternational Centre for Diarrhoeal Disease Research, BangladeshRan2022 to 2022Enrolled75ConditionsCytokine Storm, COVID-19
3 · Its place in the literature

Who cites it

429 citing papers in PubMed, 6 syntheses or guidelines pooled it, 709 citations in OpenAlex.

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  10. Gut microbiota associate with disease severity and survival in idiopathic pulmonary fibrosis.American journal of respiratory and critical care medicine · 2026
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369 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Robert P DicksonDivision of Pulmonary and Critical Care Medicine, Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA.ORCID 0000-0002-6875-4277
Benjamin H SingerDivision of Pulmonary and Critical Care Medicine, Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA.
Michael W NewsteadDivision of Pulmonary and Critical Care Medicine, Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA.
Nicole R FalkowskiDivision of Pulmonary and Critical Care Medicine, Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA.
John R Erb-DownwardDivision of Pulmonary and Critical Care Medicine, Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA.
Theodore J StandifordDivision of Pulmonary and Critical Care Medicine, Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA.
Gary B HuffnagleDivision of Pulmonary and Critical Care Medicine, Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA.
University of Michigan · US

Funding

Michigan Institute for Clinical and Health Research (MCHR)UL1TR000433 · NCATS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI MASHOUR, GEORGE ALEXANDER · 2012 to 2016
$49.9M
PULMONARY CELLULAR AND MOLECULAR BIOLOGY TRAININGT32HL007749 · NHLBI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI Robert Pickett Dickson · 1993 to 2026
$19.9M
SCCOR in Translational Research in Acute Lung InjuryP50HL074024 · NHLBI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI STANDIFORD, THEODORE J. · 2003 to 2007
$13.5M
Understanding the Lung Microbiome in HIV-Infected and HIV-uninfected individualsU01HL098961 · NHLBI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI BECK, JAMES M, CURTIS, JEFFREY LOUIS · 2009 to 2013
$3.6M
Clinical Centers (CC) for the NHLBI Prevention and Early Treatment of Acute LungU01HL123031 · NHLBI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI HYZY, ROBERT C, PARK, PAULINE K · 2014 to 2020
$2.6M
Novel IL-1 Family Members in Lung Innate ImmunityR01HL123515 · NHLBI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI STANDIFORD, THEODORE J. · 2015 to 2018
$2.5M
Pulmonary bacterial microbiome-epithelial cell interactions in COPDR01HL114447 · NHLBI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI HUFFNAGLE, GARY B, MARTINEZ, FERNANDO J · 2012 to 2015
$1.5M
The Role of the Microbiome in the Acute Respiratory Distress SyndromeK23HL130641 · NHLBI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI DICKSON, ROBERT PICKETT · 2016 to 2020
$859k
NCATS NIH HHS UL1 TR000433NHLBI NIH HHS K23 HL130641NHLBI NIH HHS L30 HL120241NHLBI NIH HHS P50 HL074024NHLBI NIH HHS R01 HL114447NHLBI NIH HHS R01 HL123515NHLBI NIH HHS T32 HL007749NHLBI NIH HHS U01 HL098961NHLBI NIH HHS U01 HL123031
6 · The paper itself

Abstract

Sepsis and the acute respiratory distress syndrome (ARDS) are major causes of mortality without targeted therapies. Although many experimental and clinical observations have implicated gut microbiota in the pathogenesis of these diseases, culture-based studies have failed to demonstrate translocation of bacteria to the lungs in critically ill patients. Here, we report culture-independent evidence that the lung microbiome is enriched with gut bacteria both in a murine model of sepsis and in humans with established ARDS. Following experimental sepsis, lung communities were dominated by viable gut-associated bacteria. Ecological analysis identified the lower gastrointestinal tract, rather than the upper respiratory tract, as the likely source community of post-sepsis lung bacteria. In bronchoalveolar lavage fluid from humans with ARDS, gut-specific bacteria (Bacteroides spp.) were common and abundant, undetected by culture and correlated with the intensity of systemic inflammation. Alveolar TNF-α, a key mediator of alveolar inflammation in ARDS, was significantly correlated with altered lung microbiota. Our results demonstrate that the lung microbiome is enriched with gut-associated bacteria in sepsis and ARDS, potentially representing a shared mechanism of pathogenesis in these common and lethal diseases.

Indexed as

MicrobiotaAdultAnimalsBacteriaBacteroidesBronchoalveolar Lavage FluidDisease Models, AnimalFemaleGastrointestinal TractHigh-Throughput Nucleotide SequencingHumansInflammationLungMaleMiceMiddle AgedTumor Necrosis Factor-alpha

Identifiers

PMID27670109
PMCPMC5076472
OpenAlexW2496661162

What OpenQuestion holds

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Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.