Evidence map›Paper›PMID 27668828›Full record

ArticleBiochemistry2016

A Carboxylate Shift Regulates Dioxygen Activation by the Diiron Nonheme β-Hydroxylase CmlA upon Binding of a Substrate-Loaded Nonribosomal Peptide Synthetase.

Andrew J Jasniewski, Cory J Knoot, John D Lipscomb, Lawrence Que

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biochemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
0.9field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 22 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Biosynthesis and Mechanism of Action of the Cell Wall Targeting Antibiotic Hypeptin.Angewandte Chemie (International ed. in English) · 2021
    Article
  5. Article
  6. Review
  7. Review
  8. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Andrew J Jasniewski *Department of Chemistry, University of Minnesota, Minneapolis, Minnesota 55455.
Cory J Knoot *Department of Biochemistry Molecular Biology and Biophysics, University of Minnesota, Minneapolis, Minnesota 55455.
John D LipscombDepartment of Biochemistry Molecular Biology and Biophysics, University of Minnesota, Minneapolis, Minnesota 55455.
Lawrence QueDepartment of Chemistry, University of Minnesota, Minneapolis, Minnesota 55455.
University of Minnesota · US

Funding

X-ray Absorption Spectroscopy (XAS) pp.711-759P41GM103393 · NIGMS · STANFORD UNIVERSITY · PI HODGSON, KEITH O · 2012 to 2019
$30.6M
Synthetic Models and Spectroscopy of Nonheme Diiron EnzymesR01GM038767 · NIGMS · UNIVERSITY OF MINNESOTA TWIN CITIES · PI QUE, LAWRENCE · 1987 to 2018
$5.8M
Chemistry-Biology Interface Training GrantT32GM008700 · NIGMS · UNIVERSITY OF MINNESOTA TWIN CITIES · PI DISTEFANO, MARK D · 1999 to 2018
$4.0M
Intermediates in O2 Activation by Oxygenases at Non-heme Iron CentersR35GM118030 · NIGMS · UNIVERSITY OF MINNESOTA · PI LIPSCOMB, JOHN D · 2016 to 2020
$2.7M
Roles of protein structure and diiron cluster chemistry in oxygen activationR01GM100943 · NIGMS · UNIVERSITY OF MINNESOTA · PI LIPSCOMB, JOHN D · 2012 to 2015
$1.2M
NIGMS NIH HHS P41 GM103393NIGMS NIH HHS R01 GM038767NIGMS NIH HHS R01 GM100943NIGMS NIH HHS R35 GM118030NIGMS NIH HHS T32 GM008700
6 · The paper itself

Abstract

The first step in the nonribosomal peptide synthetase (NRPS)-based biosynthesis of chloramphenicol is the β-hydroxylation of the precursor l-p-aminophenylalanine (l-PAPA) catalyzed by the monooxygenase CmlA. The active site of CmlA contains a dinuclear iron cluster that is reduced to the diferrous state (WT

Indexed as

Carboxylic AcidsCrystallography, X-RayIronKineticsMixed Function OxygenasesOxygenPeptide SynthasesSubstrate SpecificityX-Ray Absorption SpectroscopyCarboxylic AcidsIronMixed Function Oxygenasesnon-ribosomal peptide synthaseOxygenPeptide Synthases

Identifiers

PMID27668828
PMCPMC5258830
OpenAlexW2526788875

What OpenQuestion holds

Textmetadata
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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.